Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 94
Page xviii
... TRANSFER OF DNA FROM AGAROSE GELS TO SOLID SUPPORTS 9.34 Transfer of DNA to Nitrocellulose Filters 9.38 CAPILLARY TRANSFER OF DNA TO NITROCELLULOSE FILTERS 9.38 SIMULTANEOUS TRANSFER OF DNA FROM A SINGLE AGAROSE GEL TO TWO ...
... TRANSFER OF DNA FROM AGAROSE GELS TO SOLID SUPPORTS 9.34 Transfer of DNA to Nitrocellulose Filters 9.38 CAPILLARY TRANSFER OF DNA TO NITROCELLULOSE FILTERS 9.38 SIMULTANEOUS TRANSFER OF DNA FROM A SINGLE AGAROSE GEL TO TWO ...
Page 16-63
... Transfer the sample to an ice bath , and add 100 μl of ice - cold ethyl acetate . Mix the phases by vigorous vortexing . Centrifuge the mixtures at 12,000g for 3 minutes at room temperature in a microfuge . 4. Transfer 80 μl of the ...
... Transfer the sample to an ice bath , and add 100 μl of ice - cold ethyl acetate . Mix the phases by vigorous vortexing . Centrifuge the mixtures at 12,000g for 3 minutes at room temperature in a microfuge . 4. Transfer 80 μl of the ...
Page 18-64
... transfer . Furthermore , charged supports such as nylon membranes may not bind proteins of the same charge with high efficiency . Consequently , most western blotting nowadays is carried out by direct electrophoretic transfer of ...
... transfer . Furthermore , charged supports such as nylon membranes may not bind proteins of the same charge with high efficiency . Consequently , most western blotting nowadays is carried out by direct electrophoretic transfer of ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml