Molecular Cloning: A Laboratory Manual, Book 3 |
From inside the book
Results 1-3 of 78
Page 16-46
... transformation ) . 7. a . Transient expression : Harvest the cells 48-60 hours after transfection for analysis of RNA or DNA by hybridization . Newly synthesized protein may be analyzed by radioimmunoassay , by western blotting , by ...
... transformation ) . 7. a . Transient expression : Harvest the cells 48-60 hours after transfection for analysis of RNA or DNA by hybridization . Newly synthesized protein may be analyzed by radioimmunoassay , by western blotting , by ...
Page 16-47
... transformation of CHO cells by plasmid DNA , yielding approximately 15 - fold more trans- formants than calcium phosphate - DNA coprecipitation . However , there is no difference between the two methods in the efficiency of transformation ...
... transformation of CHO cells by plasmid DNA , yielding approximately 15 - fold more trans- formants than calcium phosphate - DNA coprecipitation . However , there is no difference between the two methods in the efficiency of transformation ...
Page 1-45
... Transformation of competent E. coli , 1.74–1.84 electrotransformation , 1.75 FSB buffer , 1.78 morphological , by ... Transformation of Competent E coli 1 74 Transformation of E coli by High-voltage Electroporation 75.
... Transformation of competent E. coli , 1.74–1.84 electrotransformation , 1.75 FSB buffer , 1.78 morphological , by ... Transformation of Competent E coli 1 74 Transformation of E coli by High-voltage Electroporation 75.
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
75 other sections not shown
Other editions - View all
Common terms and phrases
Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml