Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 16-17
... vectors in current use are idiosyncratic in their design and frequently do not contain either restriction sites or controlling elements that are universally useful . Construction of new recombinant vectors often requires extensive ...
... vectors in current use are idiosyncratic in their design and frequently do not contain either restriction sites or controlling elements that are universally useful . Construction of new recombinant vectors often requires extensive ...
Page 16-17
... vectors in current use are idiosyncratic in their design and frequently do not contain either restriction sites or controlling elements that are universally useful . Construction of new recombinant vectors often requires extensive ...
... vectors in current use are idiosyncratic in their design and frequently do not contain either restriction sites or controlling elements that are universally useful . Construction of new recombinant vectors often requires extensive ...
Page 16-25
... vectors ( 8–14 kb ) and the dearth of unique cloning sites , the insertion of a foreign gene into BPV vectors is not always straightforward . Most vectors contain only one or two restriction sites at which the coding sequences of ...
... vectors ( 8–14 kb ) and the dearth of unique cloning sites , the insertion of a foreign gene into BPV vectors is not always straightforward . Most vectors contain only one or two restriction sites at which the coding sequences of ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml