Molecular Cloning: A Laboratory Manual, Book 3 |
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Page B-13
... volume to 1 liter with H2O . Dispense into aliquots and sterilize by autoclaving . Dissolve 292.2 g of NaCl in 800 ml of H2O . Adjust the volume to 1 liter with H2O . Dis- pense into aliquots and sterilize by auto- claving . Dissolve ...
... volume to 1 liter with H2O . Dispense into aliquots and sterilize by autoclaving . Dissolve 292.2 g of NaCl in 800 ml of H2O . Adjust the volume to 1 liter with H2O . Dis- pense into aliquots and sterilize by auto- claving . Dissolve ...
Page E-8
... volume of fresh 5 % hypophosphorous acid and 0.12 volume of fresh 0.5 м sodium nitrite . Mix carefully . Important : Check that the pH of the solution is < 3.0 . Hypophosphorous acid is usually supplied as a 50 % solution , which is ...
... volume of fresh 5 % hypophosphorous acid and 0.12 volume of fresh 0.5 м sodium nitrite . Mix carefully . Important : Check that the pH of the solution is < 3.0 . Hypophosphorous acid is usually supplied as a 50 % solution , which is ...
Page E-16
... volume of a nucleic acid solution can be reduced significantly . This method of concentration is used to reduce the volume of dilute solutions to the point where the nucleic acid can easily be recovered by precipitation with ethanol . 1 ...
... volume of a nucleic acid solution can be reduced significantly . This method of concentration is used to reduce the volume of dilute solutions to the point where the nucleic acid can easily be recovered by precipitation with ethanol . 1 ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml