Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 53
Page 16-38
... wash them once with PBS . Resuspend the cells in 10 ml of prewarmed complete growth medium . Return the cells to the incubator for 48 hours before assaying for transient expression of transfected genes ( step 6a , page 16.36 ) or ...
... wash them once with PBS . Resuspend the cells in 10 ml of prewarmed complete growth medium . Return the cells to the incubator for 48 hours before assaying for transient expression of transfected genes ( step 6a , page 16.36 ) or ...
Page 18-23
... Wash the wells twice more with blocking buffer ( see step 4 ) . 7. Make a series of dilutions in blocking buffer of the antigen that is to be assayed . Add 50 μl of each dilution to each of two wells . Cover the plate and incubate it ...
... Wash the wells twice more with blocking buffer ( see step 4 ) . 7. Make a series of dilutions in blocking buffer of the antigen that is to be assayed . Add 50 μl of each dilution to each of two wells . Cover the plate and incubate it ...
Page 18-45
... wash protein A - antigen - antibody complexes . The aim is to displace proteins that are nonspecifically adsorbed to ... wash is carried out with NET - gel buffer supplemented with NaCl to a final concentration of 0.5 M. For the second ...
... wash protein A - antigen - antibody complexes . The aim is to displace proteins that are nonspecifically adsorbed to ... wash is carried out with NET - gel buffer supplemented with NaCl to a final concentration of 0.5 M. For the second ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
Copyright | |
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Common terms and phrases
Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml