Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 18-60
... ( WESTERN BLOTTING ) Western blotting ( Towbin et al . 1979 ; Burnette 1981 ) is to proteins what Southern blotting is to DNA . In both techniques , electrophoretically sepa- rated components are transferred from a gel to a solid support ...
... ( WESTERN BLOTTING ) Western blotting ( Towbin et al . 1979 ; Burnette 1981 ) is to proteins what Southern blotting is to DNA . In both techniques , electrophoretically sepa- rated components are transferred from a gel to a solid support ...
Page 18-63
... western blotting . The lowest amount of an average - sized protein that can be detected by western blotting is approximately 1-5 ng . Approximately 100 μg of total cellular protein can be applied to a lane in a 0.75 - mm - thick SDS ...
... western blotting . The lowest amount of an average - sized protein that can be detected by western blotting is approximately 1-5 ng . Approximately 100 μg of total cellular protein can be applied to a lane in a 0.75 - mm - thick SDS ...
Page 18-64
... western blotting nowadays is carried out by direct electrophoretic transfer of proteins from the gel to a nitrocellulose filter ( Burnette 1981 ) . Two types of electrophoresis apparatuses are available for electroblotting . In the ...
... western blotting nowadays is carried out by direct electrophoretic transfer of proteins from the gel to a nitrocellulose filter ( Burnette 1981 ) . Two types of electrophoresis apparatuses are available for electroblotting . In the ...
Contents
Selectable Markers 1 | 16-7 |
VECTOR SYSTEMS 16 | 16-17 |
DEVELOPMENT OF PLASMID CLONING VECTORS | 16-24 |
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Acad acetate acrylamide activity Adjust the volume agarose aliquots amount antibody antigen antisera aqueous assay autoclaving bacterial bacteriophage T4 BamHI Biol calcium cDNA cell lines centrifugation chloramphenicol cloned gene column containing culture deionized H₂O dhfr dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution encoding Escherichia coli ethanol ethidium bromide eukaryotic extract fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate ligation lysate mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyacrylamide polyclonal precipitate prepared promoter protoplasts radioactive radiolabeled reaction reagent recombinant remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences serum sodium sterile stock solution stored strain supernatant target protein Telephone termini transcription transfection transfer transient expression Tris Cl pH wash western blotting µg/ml