Plant Genotyping II: SNP TechnologyRobert J. Henry Since the publication of Plant Genotyping: the DNA Fingerprinting of Plants in 2001, the techniques available for plant DNA analysis have advanced considerably. Recent developments focus on high throughput methods, and generally target single nucleotide polymorphism (SNP) discovery and analysis. SNPs represent the most common form of genetic variation in both plants and animals, and play a key role in revealing the molecular mechanisms underlying traits. Plant Genotyping II: SNP Technology describes some of the import recent developments in this field, with the main focus on SNPs. Contributions cover the discovery, analysis and uses of SNPs, while also examining other approaches to plant genotyping. |
Contents
1 SNP Discovery in Plants | 1 |
2 SNPs and Their Use in Maize | 30 |
3 Rare SNP Discovery with Endonucleases | 44 |
4 Sequence Polymorphisms in the Flanking Regions of Microsatellite Markers | 68 |
5 SNP Discovery by Ecotilling Using Capillary Electrophoresis | 78 |
6 Genotyping by Allelespecific PCR | 88 |
7 The MassARRAY System for Plant Genomics | 98 |
8 Mutation Screening | 114 |
10 Functionally Associated Molecular Genetic Markers for Temperate Pasture Plant Improvement | 154 |
11 Genotyping for Rice Eating Qualities | 187 |
12 Towards Universal Loci for Plant Genotyping | 195 |
13 DNA Banks as a Resource for SNP Genotyping | 207 |
14 DNA Extraction from Plant Tissue | 219 |
15 Future Prospects for Plant Genotyping | 272 |
| 281 | |
The Future of Costeffective Plant Genotyping | 133 |
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