Nitric Oxide Synthase: Characterization and Functional AnalysisAlthough the function of nitric oxide in a regulatory capacity in the central and peripheral nervous system is widely recognized, the full scope of its actions and its interrelationships with other classes of regulatory molecules is just beginning to be comprehended. This volume contains a number of sophisticated and advanced methods essential for exploring the activity of nitric oxide in the brain. It will be a valuable tool for the established investigator and for those just entering the field.
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Contents
Enzyme Expression Purification and Gene Regulation | 123 |
Assessment of Nitric OxideMediated Functions at Cell and Organ Levels | 213 |
Index | 347 |
Color Plate Section | 355 |
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1996 by Academic Acad acid activity antibody antisense assay Biochem Biol brain buffer calcium cDNA cellular centrifugation cerebral Chem cloning concentration containing culture cyclic D. S. Bredt detection EDTA electrode endothelial cells endothelium eNOS enzyme expression GAPDH gene genomic heme hemoglobin hippocampal hippocampal slices hybridization incubated inhibition inhibitors intracellular isoforms L-arginine labeling medium membrane Methods in Neurosciences mg/ml molecular molecule mRNA NaCl NADPH Natl Nature London neurons Neurosci nitric oxide synthase nitrosation NMDA receptor nNOS Northern blot nucleotides oligonucleotide Pharmacol Physiol pipette plasmid platelets potential prepared primer probe Proc production protein purified reaction reagents redox release riboprobe RNase room temperature S. H. Snyder sample sequence signal slides sodium sodium nitroprusside solution specific staining Step stimulation studies supernatant synaptic target technique thiol tion tissue sections transcription transfection tube ug/ml vascular vector viral vivo washed
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