Nitric Oxide Synthase: Characterization and Functional AnalysisAlthough the function of nitric oxide in a regulatory capacity in the central and peripheral nervous system is widely recognized, the full scope of its actions and its interrelationships with other classes of regulatory molecules is just beginning to be comprehended. This volume contains a number of sophisticated and advanced methods essential for exploring the activity of nitric oxide in the brain. It will be a valuable tool for the established investigator and for those just entering the field.
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Page xv
... molecule and related components. This simple molecule, which acts in a fairly specific manner, is now recognized as an integral part of various homeostatic mechanisms, including the central and peripheral nervous system and ...
... molecule and related components. This simple molecule, which acts in a fairly specific manner, is now recognized as an integral part of various homeostatic mechanisms, including the central and peripheral nervous system and ...
Page 3
... molecule, can freely diffuse in and out of cells and tissues. The physicochemical properties of NO including high ... molecules with unpaired electrons (paramagnetic molecules) such as NO. Free NO is not detected by EPR because of its ...
... molecule, can freely diffuse in and out of cells and tissues. The physicochemical properties of NO including high ... molecules with unpaired electrons (paramagnetic molecules) such as NO. Free NO is not detected by EPR because of its ...
Page 4
... Molecules with localized unpaired electrons will exhibit g values higher or lower than 2.0023 so that even small shifts in the EPR spectra are significant and specific. Figure 1 is an idealized spectrum of a free unpaired electron ...
... Molecules with localized unpaired electrons will exhibit g values higher or lower than 2.0023 so that even small shifts in the EPR spectra are significant and specific. Figure 1 is an idealized spectrum of a free unpaired electron ...
Page 7
... molecules can serve as spin traps that stabilize NO for detection by EPR. Hemoglobin (Hb) is an attractive spin trap because it is an endogenous molecule that can be sampled by routine procedures in relatively large quantities, and its ...
... molecules can serve as spin traps that stabilize NO for detection by EPR. Hemoglobin (Hb) is an attractive spin trap because it is an endogenous molecule that can be sampled by routine procedures in relatively large quantities, and its ...
Page 9
... molecules. Whereas the complex in the case of erythrocytes and one of the complexes in smooth muscle cells is HbNO, the other complexes are classified as nonheme iron proteins that have not been fully B : -— I FIG. 3 Apparatus for ...
... molecules. Whereas the complex in the case of erythrocytes and one of the complexes in smooth muscle cells is HbNO, the other complexes are classified as nonheme iron proteins that have not been fully B : -— I FIG. 3 Apparatus for ...
Contents
Enzyme Expression Purification and Gene Regulation | 123 |
Assessment of Nitric OxideMediated Functions at Cell and Organ Levels | 213 |
Index | 347 |
Color Plate Section | 355 |
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Common terms and phrases
1996 by Academic Acad acid activity antibody antisense assay Biochem Biol brain buffer calcium cDNA cellular centrifugation cerebral Chem cloning concentration containing culture cyclic D. S. Bredt detection EDTA electrode endothelial cells endothelium eNOS enzyme expression GAPDH gene genomic heme hemoglobin hippocampal hippocampal slices hybridization incubated inhibition inhibitors intracellular isoforms L-arginine labeling medium membrane Methods in Neurosciences mg/ml molecular molecule mRNA NaCl NADPH Natl Nature London neurons Neurosci nitric oxide synthase nitrosation NMDA receptor nNOS Northern blot nucleotides oligonucleotide Pharmacol Physiol pipette plasmid platelets potential prepared primer probe Proc production protein purified reaction reagents redox release riboprobe RNase room temperature S. H. Snyder sample sequence signal slides sodium sodium nitroprusside solution specific staining Step stimulation studies supernatant synaptic target technique thiol tion tissue sections transcription transfection tube ug/ml vascular vector viral vivo washed
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