Nitric Oxide Synthase: Characterization and Functional AnalysisAlthough the function of nitric oxide in a regulatory capacity in the central and peripheral nervous system is widely recognized, the full scope of its actions and its interrelationships with other classes of regulatory molecules is just beginning to be comprehended. This volume contains a number of sophisticated and advanced methods essential for exploring the activity of nitric oxide in the brain. It will be a valuable tool for the established investigator and for those just entering the field.
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Page 21
... potential at which NO is oxidized is applied and kept constant. In the voltammetric mode, the current is measured while the potential is linearly scanned through the region that includes NO oxidation. Although the amperometric method is ...
... potential at which NO is oxidized is applied and kept constant. In the voltammetric mode, the current is measured while the potential is linearly scanned through the region that includes NO oxidation. Although the amperometric method is ...
Page 22
... potential required for the comparable metal or carbon electrodes. The current efficiency (analytical signal) for the reaction is high, even at the physiologic pH of pH 7.4. At an operational potential of 0.63–0.65 V, the sensor does not ...
... potential required for the comparable metal or carbon electrodes. The current efficiency (analytical signal) for the reaction is high, even at the physiologic pH of pH 7.4. At an operational potential of 0.63–0.65 V, the sensor does not ...
Page 23
... potential of 0.9 V is applied, and current (analytical signal) is measured from the electrochemical oxidation of nitric oxide on the platinum anode. The detection limit of the Clark probe is about 10 *M and can be achieved by measuring ...
... potential of 0.9 V is applied, and current (analytical signal) is measured from the electrochemical oxidation of nitric oxide on the platinum anode. The detection limit of the Clark probe is about 10 *M and can be achieved by measuring ...
Page 25
... potential generated by the photomultiplier in the form of a Sharp peak. The best method of calibration is to plot an integrated area under the peaks versus concentration. The chemiluminescence method measures only the NO present ...
... potential generated by the photomultiplier in the form of a Sharp peak. The best method of calibration is to plot an integrated area under the peaks versus concentration. The chemiluminescence method measures only the NO present ...
Page 26
... potential between 0.2 and 1.0 V (versus a Saturated calomel electrode) with a scan rate of 100 mV/sec for 10–15 scans. Dip coating the dried polymeric porphyrin/carbon fiber tip (3 times for 5 sec) in 1% Nafion in ethanol (Aldrich ...
... potential between 0.2 and 1.0 V (versus a Saturated calomel electrode) with a scan rate of 100 mV/sec for 10–15 scans. Dip coating the dried polymeric porphyrin/carbon fiber tip (3 times for 5 sec) in 1% Nafion in ethanol (Aldrich ...
Contents
Enzyme Expression Purification and Gene Regulation | 123 |
Assessment of Nitric OxideMediated Functions at Cell and Organ Levels | 213 |
Index | 347 |
Color Plate Section | 355 |
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Common terms and phrases
1996 by Academic Acad acid activity antibody antisense assay Biochem Biol brain buffer calcium cDNA cellular centrifugation cerebral Chem cloning concentration containing culture cyclic D. S. Bredt detection EDTA electrode endothelial cells endothelium eNOS enzyme expression GAPDH gene genomic heme hemoglobin hippocampal hippocampal slices hybridization incubated inhibition inhibitors intracellular isoforms L-arginine labeling medium membrane Methods in Neurosciences mg/ml molecular molecule mRNA NaCl NADPH Natl Nature London neurons Neurosci nitric oxide synthase nitrosation NMDA receptor nNOS Northern blot nucleotides oligonucleotide Pharmacol Physiol pipette plasmid platelets potential prepared primer probe Proc production protein purified reaction reagents redox release riboprobe RNase room temperature S. H. Snyder sample sequence signal slides sodium sodium nitroprusside solution specific staining Step stimulation studies supernatant synaptic target technique thiol tion tissue sections transcription transfection tube ug/ml vascular vector viral vivo washed
Popular passages
Page 151 - Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
Page 111 - Current Protocols in Molecular Biology" (FM Ausubel, R. Brent, RE Kingston, DD Moore, JG Seidman, JA Smith, and K. Struhl, eds.), p.
Page 111 - J. Sambrook, EF Fritsch, and T. Maniatis, "Molecular Cloning: A Laboratory Manual.
Page 315 - A practical method for grading the cognitive state of patients for the clinician. 196-8, 1975, with kind permission from Elsevier Science, Ltd., The Boulevard, Langford Lane, Kidlington OX5 1GB UK.
Page xii - College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606 LINDA J.
Page 342 - This work was supported by grants from the Medical Research Council of Canada and the National Cancer Institute of Canada.
Page 298 - Lipton, YB Choi, ZH Pan, SZ Lei, HSV Chen, NJ Sucher, J. Loscalzo, DJ Singel, and JS Stamler, Nature (London) 364, 626 (1993).
Page 46 - LJ Ignarro, GM Buga, KS Wood, RE Byrns, and G. Chaudhuri, Proc. Natl. Acad. Sci. USA, 84, 9265-9269 (1987).
Page xiii - Department of Internal Medicine, University of Michigan School of Medicine, Ann Arbor, Michigan...