Nitric Oxide Synthase: Characterization and Functional AnalysisAlthough the function of nitric oxide in a regulatory capacity in the central and peripheral nervous system is widely recognized, the full scope of its actions and its interrelationships with other classes of regulatory molecules is just beginning to be comprehended. This volume contains a number of sophisticated and advanced methods essential for exploring the activity of nitric oxide in the brain. It will be a valuable tool for the established investigator and for those just entering the field.
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Page 8
... Solutions in aerobic conditions (>5% oxygen atmosphere) due to preferential reaction of NO with O2 and oxidation of Hb to metHb (14). Under anaerobic conditions and <1% oxygen, deoxyHb readily forms HbNO by binding free NO without ...
... Solutions in aerobic conditions (>5% oxygen atmosphere) due to preferential reaction of NO with O2 and oxidation of Hb to metHb (14). Under anaerobic conditions and <1% oxygen, deoxyHb readily forms HbNO by binding free NO without ...
Page 9
... solution through Hb covalently attached to a solid agarose support in closed columns. Hemoglobin—agarose (Hb-agarose) bead columns are assembled as depicted in Fig. 3. In this column, the Hb can be reduced and deoxygenated just prior to ...
... solution through Hb covalently attached to a solid agarose support in closed columns. Hemoglobin—agarose (Hb-agarose) bead columns are assembled as depicted in Fig. 3. In this column, the Hb can be reduced and deoxygenated just prior to ...
Page 11
... solution. Polarized cells, such as endothelial cells, that require attachment for growth and survival may be cultured on microbeads and then introduced into EPR tubes, perfused, and analyzed directly in the tubes. We have used an EPR ...
... solution. Polarized cells, such as endothelial cells, that require attachment for growth and survival may be cultured on microbeads and then introduced into EPR tubes, perfused, and analyzed directly in the tubes. We have used an EPR ...
Page 16
... solution becomes optically transparent. At a constant concentration of N-(1-naphthyl)ethylenediamine dichloride and NO, the absorbance changes with the change of concentration of p-aminobenzenesulfonamide. The maximum absorbance is ...
... solution becomes optically transparent. At a constant concentration of N-(1-naphthyl)ethylenediamine dichloride and NO, the absorbance changes with the change of concentration of p-aminobenzenesulfonamide. The maximum absorbance is ...
Page 20
... solution can be different, especially at low concentrapurging gas (helium) sample optical ! windoW purger filter -Ü S- A / / photomultiplier ~ Af / calibrated leaks — power supply - - - - - - - - - - - *- | No.” No. 4 photon | amplifier ...
... solution can be different, especially at low concentrapurging gas (helium) sample optical ! windoW purger filter -Ü S- A / / photomultiplier ~ Af / calibrated leaks — power supply - - - - - - - - - - - *- | No.” No. 4 photon | amplifier ...
Contents
Enzyme Expression Purification and Gene Regulation | 123 |
Assessment of Nitric OxideMediated Functions at Cell and Organ Levels | 213 |
Index | 347 |
Color Plate Section | 355 |
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Common terms and phrases
1996 by Academic Acad acid activity antibody antisense assay Biochem Biol brain buffer calcium cDNA cellular centrifugation cerebral Chem cloning concentration containing culture cyclic D. S. Bredt detection EDTA electrode endothelial cells endothelium eNOS enzyme expression GAPDH gene genomic heme hemoglobin hippocampal hippocampal slices hybridization incubated inhibition inhibitors intracellular isoforms L-arginine labeling medium membrane Methods in Neurosciences mg/ml molecular molecule mRNA NaCl NADPH Natl Nature London neurons Neurosci nitric oxide synthase nitrosation NMDA receptor nNOS Northern blot nucleotides oligonucleotide Pharmacol Physiol pipette plasmid platelets potential prepared primer probe Proc production protein purified reaction reagents redox release riboprobe RNase room temperature S. H. Snyder sample sequence signal slides sodium sodium nitroprusside solution specific staining Step stimulation studies supernatant synaptic target technique thiol tion tissue sections transcription transfection tube ug/ml vascular vector viral vivo washed
Popular passages
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