Physical Principles and Techniques of Protein ChemistrySydney J. Leach Physical Principles and Techniques of Protein Chemistry Part C ... |
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Page 123
... spectrum obeyed Beer's law , so that the molar difference absorption coefficient was concentra- tion - independent ; ( 3 ) solvent perturbation experiments using methanol , ethylene glycol , polyethylene glycol , and glycerol ...
... spectrum obeyed Beer's law , so that the molar difference absorption coefficient was concentra- tion - independent ; ( 3 ) solvent perturbation experiments using methanol , ethylene glycol , polyethylene glycol , and glycerol ...
Page 124
... spectrum . Sketch of a hypothetical absorption spectrum e ( ) and the same spectrum shifted to longer wavelength without change in intensity ( λ — Aλ ) . When the unshifted spectrum is subtracted from the shifted spectrum , the difference ...
... spectrum . Sketch of a hypothetical absorption spectrum e ( ) and the same spectrum shifted to longer wavelength without change in intensity ( λ — Aλ ) . When the unshifted spectrum is subtracted from the shifted spectrum , the difference ...
Page 155
... absorption between sample and reference solutions is very small , highly absorbing solutions may be used b C H H b d ... spectrum viewed by the slit . The slit width extends from a to b in each diagram . The measured value of the absorbance , ...
... absorption between sample and reference solutions is very small , highly absorbing solutions may be used b C H H b d ... spectrum viewed by the slit . The slit width extends from a to b in each diagram . The measured value of the absorbance , ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
Dielectric Properties of Proteins | 7 |
Copyright | |
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absorption absorption spectrum amino acids applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann cell Chem chromophores coefficient components concentration curve Debye denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental film fluorescence fraction frequency gel filtration gradient groups instrument intensity interactions ionic strength ions lens light linear macromolecules magnification measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine values wavelength Weber Winzor zone