Physical Principles and Techniques of Protein ChemistrySydney J. Leach Physical Principles and Techniques of Protein Chemistry Part C ... |
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Page 151
... determined mainly from the absorption near 295 mp , where the ab- sorption of cystine is less , and that of ionized tyrosine more , than at 280 mμ . ) The method of Edelhoch is better suited for the determination of tryptophan MTIP = 10 ...
... determined mainly from the absorption near 295 mp , where the ab- sorption of cystine is less , and that of ionized tyrosine more , than at 280 mμ . ) The method of Edelhoch is better suited for the determination of tryptophan MTIP = 10 ...
Page 354
... determined by dif- ference in the Helipot readings . The time constant of the detecting system is determined mostly by the distributed capacitance and the anode resistance in the photo- multiplier circuit . It is essential to reduce the ...
... determined by dif- ference in the Helipot readings . The time constant of the detecting system is determined mostly by the distributed capacitance and the anode resistance in the photo- multiplier circuit . It is essential to reduce the ...
Page 483
... determined readily from Eq . ( 8 ) , and hence quantitative interpretation is feasible if VA and VP , the respective monomer and polymer elution volumes , are also known ( Ackers and Thompson , 1965 ) . V is determined from each of a ...
... determined readily from Eq . ( 8 ) , and hence quantitative interpretation is feasible if VA and VP , the respective monomer and polymer elution volumes , are also known ( Ackers and Thompson , 1965 ) . V is determined from each of a ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
Dielectric Properties of Proteins | 7 |
Copyright | |
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absorption absorption spectrum amino acids applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann cell Chem chromophores coefficient components concentration curve Debye denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental film fluorescence fraction frequency gel filtration gradient groups instrument intensity interactions ionic strength ions lens light linear macromolecules magnification measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine values wavelength Weber Winzor zone