Physical Principles and Techniques of Protein ChemistrySydney J. Leach Physical Principles and Techniques of Protein Chemistry Part C ... |
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Page 12
... film should be hydrophilic , in order to allow the specimen to spread out evenly . The substrate film which is in almost universal use at the present time is that of evaporated carbon . The carbon is deposited on a thin plastic film ...
... film should be hydrophilic , in order to allow the specimen to spread out evenly . The substrate film which is in almost universal use at the present time is that of evaporated carbon . The carbon is deposited on a thin plastic film ...
Page 13
... film ; in practice , however , these particles always seem to be destroyed rapidly by the electron beam . The formation across small holes of films of stain in which particles are embedded is discussed below in Section III , B . Lithium ...
... film ; in practice , however , these particles always seem to be destroyed rapidly by the electron beam . The formation across small holes of films of stain in which particles are embedded is discussed below in Section III , B . Lithium ...
Page 32
... films so that a continuous film of negative stain forms across the hole . Particles , such as the ribosomes studied by those authors , can then be suspended in a continuous film of stain , but without the support of an underlying carbon ...
... films so that a continuous film of negative stain forms across the hole . Particles , such as the ribosomes studied by those authors , can then be suspended in a continuous film of stain , but without the support of an underlying carbon ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
Dielectric Properties of Proteins | 7 |
Copyright | |
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absorption absorption spectrum amino acids applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann cell Chem chromophores coefficient components concentration curve Debye denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental film fluorescence fraction frequency gel filtration gradient groups instrument intensity interactions ionic strength ions lens light linear macromolecules magnification measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine values wavelength Weber Winzor zone