Physical Principles and Techniques of Protein ChemistrySydney J. Leach Physical Principles and Techniques of Protein Chemistry Part C ... |
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Page 194
... spectra which deviate from true spectra due to the wavelength - dependent variation of detector re- sponse and photon output of the light source . Since no two instruments are exactly alike , apparent spectra obtained with different ...
... spectra which deviate from true spectra due to the wavelength - dependent variation of detector re- sponse and photon output of the light source . Since no two instruments are exactly alike , apparent spectra obtained with different ...
Page 195
Sydney J. Leach. ( Lippert , 1957 ) . An example showing corrected spectra is given in Fig . 7 , which shows data for reduced nicotinamide adenine dinucleotide ( NADH ) . Corrected spectra may be expressed in other units . The wavenumber ...
Sydney J. Leach. ( Lippert , 1957 ) . An example showing corrected spectra is given in Fig . 7 , which shows data for reduced nicotinamide adenine dinucleotide ( NADH ) . Corrected spectra may be expressed in other units . The wavenumber ...
Page 198
... spectra are scanned into the ultraviolet , the decreasing energy of the xenon lamp is compensated for by increasingly large slit widths . The Turner Model 210 " Spectro " ( G. K. Turner Associates , Palo Alto , Calif . ) employs a ...
... spectra are scanned into the ultraviolet , the decreasing energy of the xenon lamp is compensated for by increasingly large slit widths . The Turner Model 210 " Spectro " ( G. K. Turner Associates , Palo Alto , Calif . ) employs a ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
Dielectric Properties of Proteins | 7 |
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absorption absorption spectrum amino acids applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann cell Chem chromophores coefficient components concentration curve Debye denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental film fluorescence fraction frequency gel filtration gradient groups instrument intensity interactions ionic strength ions lens light linear macromolecules magnification measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine values wavelength Weber Winzor zone