Physical Principles and Techniques of Protein ChemistrySydney J. Leach Physical Principles and Techniques of Protein Chemistry Part C ... |
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Page 111
... tion by Polariza- tion by Chro- chromophore transi- Orienta- perma- Vibrational mo- Sol- dipole Case phore vent ' moment tion dipole effects tion nent Net structure dipole shift is I N N None Red None None Red Retained II N P None Red ...
... tion by Polariza- tion by Chro- chromophore transi- Orienta- perma- Vibrational mo- Sol- dipole Case phore vent ' moment tion dipole effects tion nent Net structure dipole shift is I N N None Red None None Red Retained II N P None Red ...
Page 380
... tion of Eq . ( 10 ) which gives the gradient of refractive index as a func- tion of x and t . It will be recalled that the concentration Co of macro- molecular ions in the dialyzed solution used to form the initial boundary is given by ...
... tion of Eq . ( 10 ) which gives the gradient of refractive index as a func- tion of x and t . It will be recalled that the concentration Co of macro- molecular ions in the dialyzed solution used to form the initial boundary is given by ...
Page 516
... tion , 112-115 Exclusion chromatography , see Gel filtra- tion Exposure , of groups in proteins , see also individual groups of chromophores , 108-109 , 114 , 122–123 , 129-148 of tryptophan , 134-139 , 141-142 , 221 , 225-231 , 234-235 ...
... tion , 112-115 Exclusion chromatography , see Gel filtra- tion Exposure , of groups in proteins , see also individual groups of chromophores , 108-109 , 114 , 122–123 , 129-148 of tryptophan , 134-139 , 141-142 , 221 , 225-231 , 234-235 ...
Contents
Electron Microscopy | 2 |
Ultraviolet Absorption | 3 |
Dielectric Properties of Proteins | 7 |
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absorption absorption spectrum amino acids applied atoms axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann cell Chem chromophores coefficient components concentration curve Debye denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission enzyme equation equilibrium excitation experimental film fluorescence fraction frequency gel filtration gradient groups instrument intensity interactions ionic strength ions lens light linear macromolecules magnification measured method micrographs migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic photomultiplier Phys plot polarization polymer produced protein quantum yield ratio reaction relaxation residues resolution resolving power ribonuclease scattering shadow shown in Fig solution solvent specimen spectra structure studies technique temperature theoretical theory tion tryptophan tyrosine values wavelength Weber Winzor zone