In Vitro Biological Systems: Methods in Toxicology, Vol. 1, Volume 1Charles A. Tyson, John M. Frazier Methods in Toxicology, Volume 1: In Vitro Biological Systems, Part A provides basic techniques employed by widely recognized scientists to prepare and maintain the biological components of in vitro model systems. The book discusses the in vitro models of neural and neuromuscular systems; ocular system; respiratory system; cardiovascular system; and gastrointestinal system. The text also describes liver slices; liver hepatocytes; other liver cell systems; proximal tubule fragments; kidney cell culture; reproductive and developmental systems; immune system; and skin. Pharmacologists, toxicologists, cell biologists, physiologists, immunotoxicologists, and molecular toxicologists will find the book invaluable. |
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Page vi
... Cells Jacob N. Finkelstein 11. Isolation of Nonciliated Bronchiolar (Clara) Epithelial Cells from Mouse Lung Alvin M. Malkinson, Francis B. Miley, Christine H. Chichester, and Charles G. Plopper 12. Rat Tracheal Epithelial Cell Cultures ...
... Cells Jacob N. Finkelstein 11. Isolation of Nonciliated Bronchiolar (Clara) Epithelial Cells from Mouse Lung Alvin M. Malkinson, Francis B. Miley, Christine H. Chichester, and Charles G. Plopper 12. Rat Tracheal Epithelial Cell Cultures ...
Page 61
... cells can then be used to document the loss of neurons following exposure to neurotoxins. MATERIALS AND REAGENTS ... Clara, CA, 680-3017IV) is thawed slowly in the cold (5°C), 5-ug aliquots are prepared in 2-ml polypropylene tubes and ...
... cells can then be used to document the loss of neurons following exposure to neurotoxins. MATERIALS AND REAGENTS ... Clara, CA, 680-3017IV) is thawed slowly in the cold (5°C), 5-ug aliquots are prepared in 2-ml polypropylene tubes and ...
Page 115
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Contents
1 | |
94 | |
RESPIRATORY SYSTEM | 110 |
CARDIOVASCULAR SYSTEM | 147 |
GASTROINTESTINAL SYSTEM | 182 |
LIVER SLICES | 222 |
LIVER HEPATOCYTES | 231 |
LIVER OTHER CELL SYSTEMS | 279 |
KIDNEY PROXIMAL TUBULE FRAGMENTS | 330 |
KIDNEY CELL CULTURE | 366 |
REPRODUCTIVE AND DEVELOPMENTAL SYSTEMS | 420 |
IMMUNE SYSTEM | 455 |
SKIN | 504 |
Index | 531 |
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Common terms and phrases
1993 by Academic Academic Press acid activity aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula cell cultures cell suspension cell types cellular centrifuge tube chemical Clara cells coculture collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation enzyme epithelial cells ethanol explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth HBSS hepatocytes HEPES human incubated isolated kidney Kupffer cells laboratory layer lipocytes liver lymphocytes macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurons Pasteur pipette pellet Percoll perfusion petri dish Pharmacol Physiol pipette plastic plates preparation procedure protein proximal tubule rabbit reaggregates receptor removed renal resuspended RPMI scissors Sertoli cells Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells ug/ml vessel viability vitro vivo washed xenobiotics