In Vitro Biological Systems: Methods in Toxicology, Vol. 1, Volume 1Charles A. Tyson, John M. Frazier Methods in Toxicology, Volume 1: In Vitro Biological Systems, Part A provides basic techniques employed by widely recognized scientists to prepare and maintain the biological components of in vitro model systems. The book discusses the in vitro models of neural and neuromuscular systems; ocular system; respiratory system; cardiovascular system; and gastrointestinal system. The text also describes liver slices; liver hepatocytes; other liver cell systems; proximal tubule fragments; kidney cell culture; reproductive and developmental systems; immune system; and skin. Pharmacologists, toxicologists, cell biologists, physiologists, immunotoxicologists, and molecular toxicologists will find the book invaluable. |
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Page 3
... beaker of ACSF kept at 32°C by a surrounding water bath. The ACSF is continually bubbled with 95% O2/5% CO2 at a rate sufficient for fluid circulation but not so fast as to cause mechanical disturbance of the tissue. Experimental ...
... beaker of ACSF kept at 32°C by a surrounding water bath. The ACSF is continually bubbled with 95% O2/5% CO2 at a rate sufficient for fluid circulation but not so fast as to cause mechanical disturbance of the tissue. Experimental ...
Page 19
... beaker containing autoclaved petroleum jelly and a sterile glass rod. Cultures are fed in groups of four. Two sterile square coverslips are placed in each of the two 20 X 150 mm petri dishes with a pair of coverslip forceps. The Square ...
... beaker containing autoclaved petroleum jelly and a sterile glass rod. Cultures are fed in groups of four. Two sterile square coverslips are placed in each of the two 20 X 150 mm petri dishes with a pair of coverslip forceps. The Square ...
Page 73
... beakers, 150 ml Plastic graduated cylinders, 100 ml Dissecting scissors Chromaffin cells are isolated from the adrenal medulla by digestion with a collagenase-DNase Solution (collagenase) mixture. Collagenase appears to the key factor ...
... beakers, 150 ml Plastic graduated cylinders, 100 ml Dissecting scissors Chromaffin cells are isolated from the adrenal medulla by digestion with a collagenase-DNase Solution (collagenase) mixture. Collagenase appears to the key factor ...
Page 74
... beaker in an incubator at 37°C for 10 min. The glands are then perfused to digest the medulla by injecting the adrenal vein with 5 ml of collagenase mixture (0.2% collagenase type I, 15 ug/ml DNase in Ca”-free Lockes buffer) and ...
... beaker in an incubator at 37°C for 10 min. The glands are then perfused to digest the medulla by injecting the adrenal vein with 5 ml of collagenase mixture (0.2% collagenase type I, 15 ug/ml DNase in Ca”-free Lockes buffer) and ...
Page 127
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Contents
1 | |
94 | |
RESPIRATORY SYSTEM | 110 |
CARDIOVASCULAR SYSTEM | 147 |
GASTROINTESTINAL SYSTEM | 182 |
LIVER SLICES | 222 |
LIVER HEPATOCYTES | 231 |
LIVER OTHER CELL SYSTEMS | 279 |
KIDNEY PROXIMAL TUBULE FRAGMENTS | 330 |
KIDNEY CELL CULTURE | 366 |
REPRODUCTIVE AND DEVELOPMENTAL SYSTEMS | 420 |
IMMUNE SYSTEM | 455 |
SKIN | 504 |
Index | 531 |
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Common terms and phrases
1993 by Academic Academic Press acid activity aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula cell cultures cell suspension cell types cellular centrifuge tube chemical Clara cells coculture collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation enzyme epithelial cells ethanol explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth HBSS hepatocytes HEPES human incubated isolated kidney Kupffer cells laboratory layer lipocytes liver lymphocytes macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurons Pasteur pipette pellet Percoll perfusion petri dish Pharmacol Physiol pipette plastic plates preparation procedure protein proximal tubule rabbit reaggregates receptor removed renal resuspended RPMI scissors Sertoli cells Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells ug/ml vessel viability vitro vivo washed xenobiotics