In Vitro Biological Systems: Methods in Toxicology, Vol. 1, Volume 1Charles A. Tyson, John M. Frazier Methods in Toxicology, Volume 1: In Vitro Biological Systems, Part A provides basic techniques employed by widely recognized scientists to prepare and maintain the biological components of in vitro model systems. The book discusses the in vitro models of neural and neuromuscular systems; ocular system; respiratory system; cardiovascular system; and gastrointestinal system. The text also describes liver slices; liver hepatocytes; other liver cell systems; proximal tubule fragments; kidney cell culture; reproductive and developmental systems; immune system; and skin. Pharmacologists, toxicologists, cell biologists, physiologists, immunotoxicologists, and molecular toxicologists will find the book invaluable. |
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Page 30
... Serum Preparation Both fetal bovine and horse sera (GIBCO) are dialyzed prior to use in culture media in order to remove high concentrations of serotonin present in serum. Sera are dialyzed using dialysis tubing with a molecular weight ...
... Serum Preparation Both fetal bovine and horse sera (GIBCO) are dialyzed prior to use in culture media in order to remove high concentrations of serotonin present in serum. Sera are dialyzed using dialysis tubing with a molecular weight ...
Page 32
... bovine serum (FBS), 2.4% (v/v) DNase solution, and 0.86% (v/v) penicillin—streptomycin solution. This solution is referred to as BME-FBS. The tiSSue is washed three times with 2 ml CMFT and Once with 1 ml of BME-FBS. After bringing the ...
... bovine serum (FBS), 2.4% (v/v) DNase solution, and 0.86% (v/v) penicillin—streptomycin solution. This solution is referred to as BME-FBS. The tiSSue is washed three times with 2 ml CMFT and Once with 1 ml of BME-FBS. After bringing the ...
Page 33
... serum instead of fetal bovine serum. The medium is changed every 2 days thereafter in the same manner. An alternative, nonenzymatic method for the preparation of dissociated cells and their reaggregation involving the serial passage of ...
... serum instead of fetal bovine serum. The medium is changed every 2 days thereafter in the same manner. An alternative, nonenzymatic method for the preparation of dissociated cells and their reaggregation involving the serial passage of ...
Page 35
... bovine serum albumin are run in parallel. After 30 min of incubation with BCA working reagent (Pierce, Rockford, IL) at 37°C, the absorbance at 562 nm is read. Measurement of endogenous GABA levels is conducted using a modification of ...
... bovine serum albumin are run in parallel. After 30 min of incubation with BCA working reagent (Pierce, Rockford, IL) at 37°C, the absorbance at 562 nm is read. Measurement of endogenous GABA levels is conducted using a modification of ...
Page 47
... bovine serum (HyClone, Logan, UT, heat-inactivate according to HyClone directions) 20 ml defined horse serum (HyClone, heat-inactivate) Bring total volume to 200 ml with MS. Store at 4°C for up to 2 weeks. Growth medium (GM) 2 ml 200 mM ...
... bovine serum (HyClone, Logan, UT, heat-inactivate according to HyClone directions) 20 ml defined horse serum (HyClone, heat-inactivate) Bring total volume to 200 ml with MS. Store at 4°C for up to 2 weeks. Growth medium (GM) 2 ml 200 mM ...
Contents
1 | |
94 | |
RESPIRATORY SYSTEM | 110 |
CARDIOVASCULAR SYSTEM | 147 |
GASTROINTESTINAL SYSTEM | 182 |
LIVER SLICES | 222 |
LIVER HEPATOCYTES | 231 |
LIVER OTHER CELL SYSTEMS | 279 |
KIDNEY PROXIMAL TUBULE FRAGMENTS | 330 |
KIDNEY CELL CULTURE | 366 |
REPRODUCTIVE AND DEVELOPMENTAL SYSTEMS | 420 |
IMMUNE SYSTEM | 455 |
SKIN | 504 |
Index | 531 |
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1993 by Academic Academic Press acid activity aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula cell cultures cell suspension cell types cellular centrifuge tube chemical Clara cells coculture collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation enzyme epithelial cells ethanol explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth HBSS hepatocytes HEPES human incubated isolated kidney Kupffer cells laboratory layer lipocytes liver lymphocytes macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurons Pasteur pipette pellet Percoll perfusion petri dish Pharmacol Physiol pipette plastic plates preparation procedure protein proximal tubule rabbit reaggregates receptor removed renal resuspended RPMI scissors Sertoli cells Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells ug/ml vessel viability vitro vivo washed xenobiotics