In Vitro Biological Systems: Methods in Toxicology, Vol. 1, Volume 1Charles A. Tyson, John M. Frazier Methods in Toxicology, Volume 1: In Vitro Biological Systems, Part A provides basic techniques employed by widely recognized scientists to prepare and maintain the biological components of in vitro model systems. The book discusses the in vitro models of neural and neuromuscular systems; ocular system; respiratory system; cardiovascular system; and gastrointestinal system. The text also describes liver slices; liver hepatocytes; other liver cell systems; proximal tubule fragments; kidney cell culture; reproductive and developmental systems; immune system; and skin. Pharmacologists, toxicologists, cell biologists, physiologists, immunotoxicologists, and molecular toxicologists will find the book invaluable. |
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Page viii
... Cortical Epithelial Cells 366 Mary L. Taub 34. Culture of Rat Kidney Proximal Tubule Epithelial Cells in Defined Medium 374 James L. Stevens and Guohong Zhang 35. Primary Culture of Rat Renal Medullary Interstitial Cells 385 Gabrielle M ...
... Cortical Epithelial Cells 366 Mary L. Taub 34. Culture of Rat Kidney Proximal Tubule Epithelial Cells in Defined Medium 374 James L. Stevens and Guohong Zhang 35. Primary Culture of Rat Renal Medullary Interstitial Cells 385 Gabrielle M ...
Page 7
... cortical and subcortical zones, laminar cortical architecture that is reminiscent of cerebellar cortex in vivo, typical cortical and subcortical neurons with appropriate interneuronal relationships, and myelinated axons (1). Some of ...
... cortical and subcortical zones, laminar cortical architecture that is reminiscent of cerebellar cortex in vivo, typical cortical and subcortical neurons with appropriate interneuronal relationships, and myelinated axons (1). Some of ...
Page 18
... cortical strip is then cross cut into 0.5 mm sections in the parasagittal plane with a second pair of scalpels. The sections are collected with pipettes into nutrient medium, and the remainder of the steps are as described for ...
... cortical strip is then cross cut into 0.5 mm sections in the parasagittal plane with a second pair of scalpels. The sections are collected with pipettes into nutrient medium, and the remainder of the steps are as described for ...
Page 19
... are presented in Figs. 1–3. The cerebellar culture shown in Fig. 1A, which is 23 days in vitro (DIV), has recognizable cortical and Figure 1 Organotypic cerebellar cultures. (A) Low-power view of a 2. Organotypic Cultures 19 COMMENTS.
... are presented in Figs. 1–3. The cerebellar culture shown in Fig. 1A, which is 23 days in vitro (DIV), has recognizable cortical and Figure 1 Organotypic cerebellar cultures. (A) Low-power view of a 2. Organotypic Cultures 19 COMMENTS.
Page 20
... cortical (Co) and deep nucleus (Nu) regions. Lamination is evident in cortex on the right-hand side and near the top of the photomicrograph. Holmes silver stain, X39. [Reproduced with permission from F. J. Seil and A. L. Leiman, Exp ...
... cortical (Co) and deep nucleus (Nu) regions. Lamination is evident in cortex on the right-hand side and near the top of the photomicrograph. Holmes silver stain, X39. [Reproduced with permission from F. J. Seil and A. L. Leiman, Exp ...
Contents
1 | |
94 | |
RESPIRATORY SYSTEM | 110 |
CARDIOVASCULAR SYSTEM | 147 |
GASTROINTESTINAL SYSTEM | 182 |
LIVER SLICES | 222 |
LIVER HEPATOCYTES | 231 |
LIVER OTHER CELL SYSTEMS | 279 |
KIDNEY PROXIMAL TUBULE FRAGMENTS | 330 |
KIDNEY CELL CULTURE | 366 |
REPRODUCTIVE AND DEVELOPMENTAL SYSTEMS | 420 |
IMMUNE SYSTEM | 455 |
SKIN | 504 |
Index | 531 |
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1993 by Academic Academic Press acid activity aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula cell cultures cell suspension cell types cellular centrifuge tube chemical Clara cells coculture collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation enzyme epithelial cells ethanol explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth HBSS hepatocytes HEPES human incubated isolated kidney Kupffer cells laboratory layer lipocytes liver lymphocytes macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurons Pasteur pipette pellet Percoll perfusion petri dish Pharmacol Physiol pipette plastic plates preparation procedure protein proximal tubule rabbit reaggregates receptor removed renal resuspended RPMI scissors Sertoli cells Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells ug/ml vessel viability vitro vivo washed xenobiotics