In Vitro Biological Systems: Methods in Toxicology, Vol. 1, Volume 1Charles A. Tyson, John M. Frazier Methods in Toxicology, Volume 1: In Vitro Biological Systems, Part A provides basic techniques employed by widely recognized scientists to prepare and maintain the biological components of in vitro model systems. The book discusses the in vitro models of neural and neuromuscular systems; ocular system; respiratory system; cardiovascular system; and gastrointestinal system. The text also describes liver slices; liver hepatocytes; other liver cell systems; proximal tubule fragments; kidney cell culture; reproductive and developmental systems; immune system; and skin. Pharmacologists, toxicologists, cell biologists, physiologists, immunotoxicologists, and molecular toxicologists will find the book invaluable. |
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Page 15
... media components are sterile, and antibiotics are not added. In most instances, the media used during explantation are the same as those used for culture maintenance. The medium that we currently use for cerebellar cultures is composed ...
... media components are sterile, and antibiotics are not added. In most instances, the media used during explantation are the same as those used for culture maintenance. The medium that we currently use for cerebellar cultures is composed ...
Page 27
... Cultures. for. Neurotoxicological. Studies. Alfred Heller, Lisa Won, Hyung Choi ... culture system, first described by Moscona (1) and later applied by DeLong ... medium in flasks which are rotated in an incubator. Over the next 24 hr, the ...
... Cultures. for. Neurotoxicological. Studies. Alfred Heller, Lisa Won, Hyung Choi ... culture system, first described by Moscona (1) and later applied by DeLong ... medium in flasks which are rotated in an incubator. Over the next 24 hr, the ...
Page 30
... culture media in order to remove high concentrations of serotonin present in serum. Sera are dialyzed using dialysis ... Medium for Culturing Reaggregates Medium is prepared fresh before use and consists of the following: 100 ml BME, 10 ...
... culture media in order to remove high concentrations of serotonin present in serum. Sera are dialyzed using dialysis ... Medium for Culturing Reaggregates Medium is prepared fresh before use and consists of the following: 100 ml BME, 10 ...
Page 33
... culture flask is tilted and the reaggregates are allowed to settle by gravitation. All but 0.5 ml of the medium is removed and replaced with 3.0 ml fresh medium containing horse serum instead of fetal bovine serum. The medium is changed ...
... culture flask is tilted and the reaggregates are allowed to settle by gravitation. All but 0.5 ml of the medium is removed and replaced with 3.0 ml fresh medium containing horse serum instead of fetal bovine serum. The medium is changed ...
Page 35
... culture medium. The specific methods for evaluation are illustrated for the dopaminergic and serotonergic systems but should be applicable to any neurochemical system by use of appropriate biochemical and immunocytochemical methods ...
... culture medium. The specific methods for evaluation are illustrated for the dopaminergic and serotonergic systems but should be applicable to any neurochemical system by use of appropriate biochemical and immunocytochemical methods ...
Contents
1 | |
94 | |
RESPIRATORY SYSTEM | 110 |
CARDIOVASCULAR SYSTEM | 147 |
GASTROINTESTINAL SYSTEM | 182 |
LIVER SLICES | 222 |
LIVER HEPATOCYTES | 231 |
LIVER OTHER CELL SYSTEMS | 279 |
KIDNEY PROXIMAL TUBULE FRAGMENTS | 330 |
KIDNEY CELL CULTURE | 366 |
REPRODUCTIVE AND DEVELOPMENTAL SYSTEMS | 420 |
IMMUNE SYSTEM | 455 |
SKIN | 504 |
Index | 531 |
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Common terms and phrases
1993 by Academic Academic Press acid activity aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula cell cultures cell suspension cell types cellular centrifuge tube chemical Clara cells coculture collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation enzyme epithelial cells ethanol explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth HBSS hepatocytes HEPES human incubated isolated kidney Kupffer cells laboratory layer lipocytes liver lymphocytes macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurons Pasteur pipette pellet Percoll perfusion petri dish Pharmacol Physiol pipette plastic plates preparation procedure protein proximal tubule rabbit reaggregates receptor removed renal resuspended RPMI scissors Sertoli cells Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells ug/ml vessel viability vitro vivo washed xenobiotics