In Vitro Biological Systems: Methods in Toxicology, Vol. 1, Volume 1Charles A. Tyson, John M. Frazier Methods in Toxicology, Volume 1: In Vitro Biological Systems, Part A provides basic techniques employed by widely recognized scientists to prepare and maintain the biological components of in vitro model systems. The book discusses the in vitro models of neural and neuromuscular systems; ocular system; respiratory system; cardiovascular system; and gastrointestinal system. The text also describes liver slices; liver hepatocytes; other liver cell systems; proximal tubule fragments; kidney cell culture; reproductive and developmental systems; immune system; and skin. Pharmacologists, toxicologists, cell biologists, physiologists, immunotoxicologists, and molecular toxicologists will find the book invaluable. |
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Page 73
... digestion with a collagenase-DNase Solution (collagenase) mixture. Collagenase appears to the key factor in obtaining a large number of viable cells; the DNase is required to prevent cell clumping during the digestion. Lots of ...
... digestion with a collagenase-DNase Solution (collagenase) mixture. Collagenase appears to the key factor in obtaining a large number of viable cells; the DNase is required to prevent cell clumping during the digestion. Lots of ...
Page 74
... Digestion by Collagenase The glands are first perfused with Lockes buffer by using a 5-ml syringe (without needle) ... digested at 37°C on a magnetic stirrer with moderate stirring for 30 min. The cell suspension is then filtered using a ...
... Digestion by Collagenase The glands are first perfused with Lockes buffer by using a 5-ml syringe (without needle) ... digested at 37°C on a magnetic stirrer with moderate stirring for 30 min. The cell suspension is then filtered using a ...
Page 76
... digestion is complete when the medulla appears flaccid and is easy to remove from the cortex. Incomplete digestion of the medulla results in a decrease in the final yield of cells obtained. There is a fair amount of variability ...
... digestion is complete when the medulla appears flaccid and is easy to remove from the cortex. Incomplete digestion of the medulla results in a decrease in the final yield of cells obtained. There is a fair amount of variability ...
Page 104
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Page 119
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Contents
1 | |
94 | |
RESPIRATORY SYSTEM | 110 |
CARDIOVASCULAR SYSTEM | 147 |
GASTROINTESTINAL SYSTEM | 182 |
LIVER SLICES | 222 |
LIVER HEPATOCYTES | 231 |
LIVER OTHER CELL SYSTEMS | 279 |
KIDNEY PROXIMAL TUBULE FRAGMENTS | 330 |
KIDNEY CELL CULTURE | 366 |
REPRODUCTIVE AND DEVELOPMENTAL SYSTEMS | 420 |
IMMUNE SYSTEM | 455 |
SKIN | 504 |
Index | 531 |
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Common terms and phrases
1993 by Academic Academic Press acid activity aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula cell cultures cell suspension cell types cellular centrifuge tube chemical Clara cells coculture collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation enzyme epithelial cells ethanol explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth HBSS hepatocytes HEPES human incubated isolated kidney Kupffer cells laboratory layer lipocytes liver lymphocytes macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurons Pasteur pipette pellet Percoll perfusion petri dish Pharmacol Physiol pipette plastic plates preparation procedure protein proximal tubule rabbit reaggregates receptor removed renal resuspended RPMI scissors Sertoli cells Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells ug/ml vessel viability vitro vivo washed xenobiotics