In Vitro Biological Systems: Methods in Toxicology, Vol. 1, Volume 1Charles A. Tyson, John M. Frazier Methods in Toxicology, Volume 1: In Vitro Biological Systems, Part A provides basic techniques employed by widely recognized scientists to prepare and maintain the biological components of in vitro model systems. The book discusses the in vitro models of neural and neuromuscular systems; ocular system; respiratory system; cardiovascular system; and gastrointestinal system. The text also describes liver slices; liver hepatocytes; other liver cell systems; proximal tubule fragments; kidney cell culture; reproductive and developmental systems; immune system; and skin. Pharmacologists, toxicologists, cell biologists, physiologists, immunotoxicologists, and molecular toxicologists will find the book invaluable. |
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Page 11
... fetal calf serum, obtained from various commercial sources, and Simms' X-7 balanced Salt Solution (BSS) prepared from analyzed or certified reagent-grade chemicals in distilled water at the following final concentrations (g/liter): NaCl ...
... fetal calf serum, obtained from various commercial sources, and Simms' X-7 balanced Salt Solution (BSS) prepared from analyzed or certified reagent-grade chemicals in distilled water at the following final concentrations (g/liter): NaCl ...
Page 14
... fetal calf serum. The coverslips are stored in this form at room temperature for 24–48 hr, after which they are used to set up cultures. Coverslips prepared in this way can be stored at refrigerator temperature for up to 2 weeks before ...
... fetal calf serum. The coverslips are stored in this form at room temperature for 24–48 hr, after which they are used to set up cultures. Coverslips prepared in this way can be stored at refrigerator temperature for up to 2 weeks before ...
Page 15
... fetal calf serum. The L-glutamine, which is supplied frozen and in 100X concentration (200 mM), is thawed, and 1 ml is added to 100 ml of Eagle's MEM for a final concentration of 2 mM. The HEPES buffer is supplied in a 1 M concentration ...
... fetal calf serum. The L-glutamine, which is supplied frozen and in 100X concentration (200 mM), is thawed, and 1 ml is added to 100 ml of Eagle's MEM for a final concentration of 2 mM. The HEPES buffer is supplied in a 1 M concentration ...
Page 16
... fetal or neonatal donor animals are optimal for different levels of the nervous system. Newborn (within 24 hr after birth) mice or rats are best for cerebellar cultures; cerebral neocortex cultures can be prepared from animals ranging ...
... fetal or neonatal donor animals are optimal for different levels of the nervous system. Newborn (within 24 hr after birth) mice or rats are best for cerebellar cultures; cerebral neocortex cultures can be prepared from animals ranging ...
Page 18
... fetus, lying on its side in a petri dish with BSS, is decapitated by crosscutting with scalpel blades. This is followed by making a cut through the ventral plane of the body under the spinal column, beginning at the neck and extending ...
... fetus, lying on its side in a petri dish with BSS, is decapitated by crosscutting with scalpel blades. This is followed by making a cut through the ventral plane of the body under the spinal column, beginning at the neck and extending ...
Contents
1 | |
94 | |
RESPIRATORY SYSTEM | 110 |
CARDIOVASCULAR SYSTEM | 147 |
GASTROINTESTINAL SYSTEM | 182 |
LIVER SLICES | 222 |
LIVER HEPATOCYTES | 231 |
LIVER OTHER CELL SYSTEMS | 279 |
KIDNEY PROXIMAL TUBULE FRAGMENTS | 330 |
KIDNEY CELL CULTURE | 366 |
REPRODUCTIVE AND DEVELOPMENTAL SYSTEMS | 420 |
IMMUNE SYSTEM | 455 |
SKIN | 504 |
Index | 531 |
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Common terms and phrases
1993 by Academic Academic Press acid activity aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula cell cultures cell suspension cell types cellular centrifuge tube chemical Clara cells coculture collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation enzyme epithelial cells ethanol explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth HBSS hepatocytes HEPES human incubated isolated kidney Kupffer cells laboratory layer lipocytes liver lymphocytes macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurons Pasteur pipette pellet Percoll perfusion petri dish Pharmacol Physiol pipette plastic plates preparation procedure protein proximal tubule rabbit reaggregates receptor removed renal resuspended RPMI scissors Sertoli cells Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells ug/ml vessel viability vitro vivo washed xenobiotics