In Vitro Biological Systems: Methods in Toxicology, Vol. 1, Volume 1Charles A. Tyson, John M. Frazier Methods in Toxicology, Volume 1: In Vitro Biological Systems, Part A provides basic techniques employed by widely recognized scientists to prepare and maintain the biological components of in vitro model systems. The book discusses the in vitro models of neural and neuromuscular systems; ocular system; respiratory system; cardiovascular system; and gastrointestinal system. The text also describes liver slices; liver hepatocytes; other liver cell systems; proximal tubule fragments; kidney cell culture; reproductive and developmental systems; immune system; and skin. Pharmacologists, toxicologists, cell biologists, physiologists, immunotoxicologists, and molecular toxicologists will find the book invaluable. |
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Page 1
... 1993 by Academic Press, Inc. All rights of reproduction in any form reserved. 1 NaH2PO4, 1.25 NaHCO3, 24 CaCl2, 1.2 MgSO4, 1.2 Glucose or SECTION I: NEURAL AND NEUROMUSCULAR SYSTEMS CHAPTER 1. HIPPOCAMPAL SLICES INTRODUCTION MATERIALS.
... 1993 by Academic Press, Inc. All rights of reproduction in any form reserved. 1 NaH2PO4, 1.25 NaHCO3, 24 CaCl2, 1.2 MgSO4, 1.2 Glucose or SECTION I: NEURAL AND NEUROMUSCULAR SYSTEMS CHAPTER 1. HIPPOCAMPAL SLICES INTRODUCTION MATERIALS.
Page 2
... Glucose or dextrose, 10 ACSF should be prepared fresh daily from the following stock solutions. The stock solutions can be kept refrigerated for several weeks. Solution A: 1 M dextrose Solution B: 240 mM CaCl2 Solution C: 240 mM MgSO ...
... Glucose or dextrose, 10 ACSF should be prepared fresh daily from the following stock solutions. The stock solutions can be kept refrigerated for several weeks. Solution A: 1 M dextrose Solution B: 240 mM CaCl2 Solution C: 240 mM MgSO ...
Page 46
... Glucose—bicarbonate stock 13.33 g sodium bicarbonate METHODS IN TOXICOLOGY, Volume 1A 46 Copyright © 1993 by Academic Press, Inc. All rights of reproduction in any form reserved. 22.22 g glucose 500 ml Sterile water Filter, store at ...
... Glucose—bicarbonate stock 13.33 g sodium bicarbonate METHODS IN TOXICOLOGY, Volume 1A 46 Copyright © 1993 by Academic Press, Inc. All rights of reproduction in any form reserved. 22.22 g glucose 500 ml Sterile water Filter, store at ...
Page 47
... glucose—bicarbonate stock (above) The additional water from the glucose-bicarbonate stock compensates for the osmotic load associated with the supplemental glucose (25 mM total) and the loss of water from the cultures by evaporation ...
... glucose—bicarbonate stock (above) The additional water from the glucose-bicarbonate stock compensates for the osmotic load associated with the supplemental glucose (25 mM total) and the loss of water from the cultures by evaporation ...
Page 48
... glucose (15 mM) 47.7 g HEPES (acid; 20 mM) 4.0 g NaOH (10 mM) 20 ml 0.5% phenol red Combine in water for a total volume of 1 liter. Filter and store at 4°C for up to 12 months. Dilute to 1X with sterile water before use. 10X Balanced ...
... glucose (15 mM) 47.7 g HEPES (acid; 20 mM) 4.0 g NaOH (10 mM) 20 ml 0.5% phenol red Combine in water for a total volume of 1 liter. Filter and store at 4°C for up to 12 months. Dilute to 1X with sterile water before use. 10X Balanced ...
Contents
1 | |
94 | |
RESPIRATORY SYSTEM | 110 |
CARDIOVASCULAR SYSTEM | 147 |
GASTROINTESTINAL SYSTEM | 182 |
LIVER SLICES | 222 |
LIVER HEPATOCYTES | 231 |
LIVER OTHER CELL SYSTEMS | 279 |
KIDNEY PROXIMAL TUBULE FRAGMENTS | 330 |
KIDNEY CELL CULTURE | 366 |
REPRODUCTIVE AND DEVELOPMENTAL SYSTEMS | 420 |
IMMUNE SYSTEM | 455 |
SKIN | 504 |
Index | 531 |
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1993 by Academic Academic Press acid activity aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula cell cultures cell suspension cell types cellular centrifuge tube chemical Clara cells coculture collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation enzyme epithelial cells ethanol explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth HBSS hepatocytes HEPES human incubated isolated kidney Kupffer cells laboratory layer lipocytes liver lymphocytes macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurons Pasteur pipette pellet Percoll perfusion petri dish Pharmacol Physiol pipette plastic plates preparation procedure protein proximal tubule rabbit reaggregates receptor removed renal resuspended RPMI scissors Sertoli cells Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells ug/ml vessel viability vitro vivo washed xenobiotics