In Vitro Biological Systems: Methods in Toxicology, Vol. 1, Volume 1Charles A. Tyson, John M. Frazier Methods in Toxicology, Volume 1: In Vitro Biological Systems, Part A provides basic techniques employed by widely recognized scientists to prepare and maintain the biological components of in vitro model systems. The book discusses the in vitro models of neural and neuromuscular systems; ocular system; respiratory system; cardiovascular system; and gastrointestinal system. The text also describes liver slices; liver hepatocytes; other liver cell systems; proximal tubule fragments; kidney cell culture; reproductive and developmental systems; immune system; and skin. Pharmacologists, toxicologists, cell biologists, physiologists, immunotoxicologists, and molecular toxicologists will find the book invaluable. |
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Results 1-5 of 83
Page 15
... growth factor (NGF; Collaborative Research, Lexington, MA) during the first 5 days in vitro. The NGF is not included in subsequent feedings because it is toxic to the spinal cord portion of the culture when applied chronically. Chick ...
... growth factor (NGF; Collaborative Research, Lexington, MA) during the first 5 days in vitro. The NGF is not included in subsequent feedings because it is toxic to the spinal cord portion of the culture when applied chronically. Chick ...
Page 47
... Growth medium (GM) 2 ml 200 mM glutamine 20 ml defined horse serum (HyClone, heat-inactivate) Bring total volume to 200 ml with MS. Store at 4°C for up to 2 weeks. Cytosine arabinoside (ARA-C) 0.010 g cytosine arabinofuranoside (Sigma C ...
... Growth medium (GM) 2 ml 200 mM glutamine 20 ml defined horse serum (HyClone, heat-inactivate) Bring total volume to 200 ml with MS. Store at 4°C for up to 2 weeks. Cytosine arabinoside (ARA-C) 0.010 g cytosine arabinofuranoside (Sigma C ...
Page 50
... growth factor (Sigma E4127, 10 ng/ml final concentration) to the PM and mix. 4. Plate the cells on tissue culture-treated 24-well plates (Falcon Primaria No. 3847) by pipetting 400 ul in each well (a 5-ml combitip on an Eppendorf repeat ...
... growth factor (Sigma E4127, 10 ng/ml final concentration) to the PM and mix. 4. Plate the cells on tissue culture-treated 24-well plates (Falcon Primaria No. 3847) by pipetting 400 ul in each well (a 5-ml combitip on an Eppendorf repeat ...
Page 53
... growth medium by aspirating the volume in each well down to 125 pul and adding 750 ul of HCSS with a repeat pipettor. This step is repeated a second time; then toxic exposure is initiated by aspirating the volume down to 125 ul and ...
... growth medium by aspirating the volume in each well down to 125 pul and adding 750 ul of HCSS with a repeat pipettor. This step is repeated a second time; then toxic exposure is initiated by aspirating the volume down to 125 ul and ...
Page 58
... growth. Alternatively, improved cell adhesion may be obtained by coating plain polystyrene surfaces with collagen, polylysine, laminin, fibronectin, or other commercially available substrates. We have found that cortical neurons attach ...
... growth. Alternatively, improved cell adhesion may be obtained by coating plain polystyrene surfaces with collagen, polylysine, laminin, fibronectin, or other commercially available substrates. We have found that cortical neurons attach ...
Contents
1 | |
94 | |
RESPIRATORY SYSTEM | 110 |
CARDIOVASCULAR SYSTEM | 147 |
GASTROINTESTINAL SYSTEM | 182 |
LIVER SLICES | 222 |
LIVER HEPATOCYTES | 231 |
LIVER OTHER CELL SYSTEMS | 279 |
KIDNEY PROXIMAL TUBULE FRAGMENTS | 330 |
KIDNEY CELL CULTURE | 366 |
REPRODUCTIVE AND DEVELOPMENTAL SYSTEMS | 420 |
IMMUNE SYSTEM | 455 |
SKIN | 504 |
Index | 531 |
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Common terms and phrases
1993 by Academic Academic Press acid activity aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula cell cultures cell suspension cell types cellular centrifuge tube chemical Clara cells coculture collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation enzyme epithelial cells ethanol explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth HBSS hepatocytes HEPES human incubated isolated kidney Kupffer cells laboratory layer lipocytes liver lymphocytes macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurons Pasteur pipette pellet Percoll perfusion petri dish Pharmacol Physiol pipette plastic plates preparation procedure protein proximal tubule rabbit reaggregates receptor removed renal resuspended RPMI scissors Sertoli cells Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells ug/ml vessel viability vitro vivo washed xenobiotics