In Vitro Biological Systems: Methods in Toxicology, Vol. 1, Volume 1Charles A. Tyson, John M. Frazier Methods in Toxicology, Volume 1: In Vitro Biological Systems, Part A provides basic techniques employed by widely recognized scientists to prepare and maintain the biological components of in vitro model systems. The book discusses the in vitro models of neural and neuromuscular systems; ocular system; respiratory system; cardiovascular system; and gastrointestinal system. The text also describes liver slices; liver hepatocytes; other liver cell systems; proximal tubule fragments; kidney cell culture; reproductive and developmental systems; immune system; and skin. Pharmacologists, toxicologists, cell biologists, physiologists, immunotoxicologists, and molecular toxicologists will find the book invaluable. |
From inside the book
Results 1-5 of 85
Page 7
... a collagen gel and inserted into test tubes with a relatively large amount of medium. The METHODS IN TOXICOLOGY, Volume 1A 7 tubes are rotated in a drum during incubation in a CHAPTER 2. OGANOTYPIC NEURAL CULTURES INTRODUCTION.
... a collagen gel and inserted into test tubes with a relatively large amount of medium. The METHODS IN TOXICOLOGY, Volume 1A 7 tubes are rotated in a drum during incubation in a CHAPTER 2. OGANOTYPIC NEURAL CULTURES INTRODUCTION.
Page 8
... incubated in the lying-drop position, thus allowing excellent gaseous exchange throughout the pe. riod of incubation. The advantages of the roller tube method are that several explants can be placed on each coverslip, and less frequent ...
... incubated in the lying-drop position, thus allowing excellent gaseous exchange throughout the pe. riod of incubation. The advantages of the roller tube method are that several explants can be placed on each coverslip, and less frequent ...
Page 17
... incubated at 35.5–36°C in a water-jacketed incubator without added moisture or gas. Gassing is not necessary, as the Maximow chamber is a closed system. Cerebral Neocortex The earliest description of the Maximow chamber method for ...
... incubated at 35.5–36°C in a water-jacketed incubator without added moisture or gas. Gassing is not necessary, as the Maximow chamber is a closed system. Cerebral Neocortex The earliest description of the Maximow chamber method for ...
Page 18
... incubated as described for cerebellum. Other levels of the nervous system can be similarly cultured in Maximow chambers, including isolated dorsal root (13), sympathetic (7,14) or trigeminal ganglia (15), hippocampus (16), striatum (17) ...
... incubated as described for cerebellum. Other levels of the nervous system can be similarly cultured in Maximow chambers, including isolated dorsal root (13), sympathetic (7,14) or trigeminal ganglia (15), hippocampus (16), striatum (17) ...
Page 31
... incubated for 20 min at 37°C in 2 ml of CMFT equilibrated with 74% N2/21% O2/5% CO2. After removing the CMFT, the tissue is incubated in CMFT containing 0.67% (w/v) trypsin (GIBCO, Cat. No. 610-5095) for 35 min at 37°C. The trypsin ...
... incubated for 20 min at 37°C in 2 ml of CMFT equilibrated with 74% N2/21% O2/5% CO2. After removing the CMFT, the tissue is incubated in CMFT containing 0.67% (w/v) trypsin (GIBCO, Cat. No. 610-5095) for 35 min at 37°C. The trypsin ...
Contents
1 | |
94 | |
RESPIRATORY SYSTEM | 110 |
CARDIOVASCULAR SYSTEM | 147 |
GASTROINTESTINAL SYSTEM | 182 |
LIVER SLICES | 222 |
LIVER HEPATOCYTES | 231 |
LIVER OTHER CELL SYSTEMS | 279 |
KIDNEY PROXIMAL TUBULE FRAGMENTS | 330 |
KIDNEY CELL CULTURE | 366 |
REPRODUCTIVE AND DEVELOPMENTAL SYSTEMS | 420 |
IMMUNE SYSTEM | 455 |
SKIN | 504 |
Index | 531 |
Other editions - View all
Common terms and phrases
1993 by Academic Academic Press acid activity aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula cell cultures cell suspension cell types cellular centrifuge tube chemical Clara cells coculture collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation enzyme epithelial cells ethanol explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth HBSS hepatocytes HEPES human incubated isolated kidney Kupffer cells laboratory layer lipocytes liver lymphocytes macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurons Pasteur pipette pellet Percoll perfusion petri dish Pharmacol Physiol pipette plastic plates preparation procedure protein proximal tubule rabbit reaggregates receptor removed renal resuspended RPMI scissors Sertoli cells Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells ug/ml vessel viability vitro vivo washed xenobiotics