In Vitro Biological Systems: Methods in Toxicology, Vol. 1, Volume 1Charles A. Tyson, John M. Frazier Methods in Toxicology, Volume 1: In Vitro Biological Systems, Part A provides basic techniques employed by widely recognized scientists to prepare and maintain the biological components of in vitro model systems. The book discusses the in vitro models of neural and neuromuscular systems; ocular system; respiratory system; cardiovascular system; and gastrointestinal system. The text also describes liver slices; liver hepatocytes; other liver cell systems; proximal tubule fragments; kidney cell culture; reproductive and developmental systems; immune system; and skin. Pharmacologists, toxicologists, cell biologists, physiologists, immunotoxicologists, and molecular toxicologists will find the book invaluable. |
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Page viii
... Isolation and Culture of Liver Epithelial Cells from Carcinogen-Treated Rats 317 Nancy L. Thompson and Nelson Fausto Section VIIA Kidney: Proximal Tubule Fragments 29. Renal Proximal Tubule Preparations Isolated from Rat or Rabbit ...
... Isolation and Culture of Liver Epithelial Cells from Carcinogen-Treated Rats 317 Nancy L. Thompson and Nelson Fausto Section VIIA Kidney: Proximal Tubule Fragments 29. Renal Proximal Tubule Preparations Isolated from Rat or Rabbit ...
Page xix
... isolated organs, tissue slices, explant cultures, and isolated primary cells as well as cell lines and subcellular fractions (e.g., microsomes, mitochondria, plasma membrane vesicles, etc.). These model systems have made major ...
... isolated organs, tissue slices, explant cultures, and isolated primary cells as well as cell lines and subcellular fractions (e.g., microsomes, mitochondria, plasma membrane vesicles, etc.). These model systems have made major ...
Page 18
... isolated spinal column with adjacent paravertebral musculature and the dorsal portions of some ribs. The block of tissue is placed ventral side down, and the skin is peeled off. A “laminectomy” (the vertebral arches have not yet fully ...
... isolated spinal column with adjacent paravertebral musculature and the dorsal portions of some ribs. The block of tissue is placed ventral side down, and the skin is peeled off. A “laminectomy” (the vertebral arches have not yet fully ...
Page 73
... isolated from the adrenal medulla by digestion with a collagenase-DNase Solution (collagenase) mixture. Collagenase ... isolation of chromaffin cells. If no established lots are available, two or three different lots are sampled by ...
... isolated from the adrenal medulla by digestion with a collagenase-DNase Solution (collagenase) mixture. Collagenase ... isolation of chromaffin cells. If no established lots are available, two or three different lots are sampled by ...
Page 75
... isolated by this method contained about 20% more catecholamine than cells isolated by the Percoll gradient method. Ten to twelve milliliters of the cell suspension in 15% Renografin is placed in 15-ml sterile, screw-capped centrifuge ...
... isolated by this method contained about 20% more catecholamine than cells isolated by the Percoll gradient method. Ten to twelve milliliters of the cell suspension in 15% Renografin is placed in 15-ml sterile, screw-capped centrifuge ...
Contents
1 | |
94 | |
RESPIRATORY SYSTEM | 110 |
CARDIOVASCULAR SYSTEM | 147 |
GASTROINTESTINAL SYSTEM | 182 |
LIVER SLICES | 222 |
LIVER HEPATOCYTES | 231 |
LIVER OTHER CELL SYSTEMS | 279 |
KIDNEY PROXIMAL TUBULE FRAGMENTS | 330 |
KIDNEY CELL CULTURE | 366 |
REPRODUCTIVE AND DEVELOPMENTAL SYSTEMS | 420 |
IMMUNE SYSTEM | 455 |
SKIN | 504 |
Index | 531 |
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Common terms and phrases
1993 by Academic Academic Press acid activity aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula cell cultures cell suspension cell types cellular centrifuge tube chemical Clara cells coculture collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation enzyme epithelial cells ethanol explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth HBSS hepatocytes HEPES human incubated isolated kidney Kupffer cells laboratory layer lipocytes liver lymphocytes macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurons Pasteur pipette pellet Percoll perfusion petri dish Pharmacol Physiol pipette plastic plates preparation procedure protein proximal tubule rabbit reaggregates receptor removed renal resuspended RPMI scissors Sertoli cells Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells ug/ml vessel viability vitro vivo washed xenobiotics