In Vitro Biological Systems: Methods in Toxicology, Vol. 1, Volume 1Charles A. Tyson, John M. Frazier Methods in Toxicology, Volume 1: In Vitro Biological Systems, Part A provides basic techniques employed by widely recognized scientists to prepare and maintain the biological components of in vitro model systems. The book discusses the in vitro models of neural and neuromuscular systems; ocular system; respiratory system; cardiovascular system; and gastrointestinal system. The text also describes liver slices; liver hepatocytes; other liver cell systems; proximal tubule fragments; kidney cell culture; reproductive and developmental systems; immune system; and skin. Pharmacologists, toxicologists, cell biologists, physiologists, immunotoxicologists, and molecular toxicologists will find the book invaluable. |
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Page xix
... membrane vesicles, etc.). These model systems have made major contributions to toxicological sciences, particularly in our understanding of mechanisms of toxicity, xenobiotic metabolism, and species differences in expressions of ...
... membrane vesicles, etc.). These model systems have made major contributions to toxicological sciences, particularly in our understanding of mechanisms of toxicity, xenobiotic metabolism, and species differences in expressions of ...
Page 4
... In either case, it is important to remove brain membranes and surface vasculature which can impede cutting. Slicing Slicing procedures are discussed above. As slices are cut,. 4 I. Neural and Neuromuscular Systems PROCEDURES.
... In either case, it is important to remove brain membranes and surface vasculature which can impede cutting. Slicing Slicing procedures are discussed above. As slices are cut,. 4 I. Neural and Neuromuscular Systems PROCEDURES.
Page 29
... membrane (i.e., Costar, Cambridge, MA, bottle-top filter, Cat. No. 8330). The sterile medium is stored at 2-8°C and has a shelf life of 2 months. Preparation of Deoxyribonuclease Solution Dissolve 100 mg of lyophilized deoxyribonuclease ...
... membrane (i.e., Costar, Cambridge, MA, bottle-top filter, Cat. No. 8330). The sterile medium is stored at 2-8°C and has a shelf life of 2 months. Preparation of Deoxyribonuclease Solution Dissolve 100 mg of lyophilized deoxyribonuclease ...
Page 30
... membrane filter and aliquot 1.25 ml into microcentrifuge tubes. The solution is stable when stored at -20°C. Serum Preparation Both fetal bovine and horse sera (GIBCO) are dialyzed prior to use in culture media in order to remove high ...
... membrane filter and aliquot 1.25 ml into microcentrifuge tubes. The solution is stable when stored at -20°C. Serum Preparation Both fetal bovine and horse sera (GIBCO) are dialyzed prior to use in culture media in order to remove high ...
Page 70
... membrane-bound granules. These dense-core granules are the most distinctive feature of these cells. Cells possess either norepinephrine (Fig. 1A) or epinephrine-containing granules (Fig. 1B). The epinephrine cells generally house more ...
... membrane-bound granules. These dense-core granules are the most distinctive feature of these cells. Cells possess either norepinephrine (Fig. 1A) or epinephrine-containing granules (Fig. 1B). The epinephrine cells generally house more ...
Contents
1 | |
94 | |
RESPIRATORY SYSTEM | 110 |
CARDIOVASCULAR SYSTEM | 147 |
GASTROINTESTINAL SYSTEM | 182 |
LIVER SLICES | 222 |
LIVER HEPATOCYTES | 231 |
LIVER OTHER CELL SYSTEMS | 279 |
KIDNEY PROXIMAL TUBULE FRAGMENTS | 330 |
KIDNEY CELL CULTURE | 366 |
REPRODUCTIVE AND DEVELOPMENTAL SYSTEMS | 420 |
IMMUNE SYSTEM | 455 |
SKIN | 504 |
Index | 531 |
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Common terms and phrases
1993 by Academic Academic Press acid activity aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula cell cultures cell suspension cell types cellular centrifuge tube chemical Clara cells coculture collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation enzyme epithelial cells ethanol explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth HBSS hepatocytes HEPES human incubated isolated kidney Kupffer cells laboratory layer lipocytes liver lymphocytes macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurons Pasteur pipette pellet Percoll perfusion petri dish Pharmacol Physiol pipette plastic plates preparation procedure protein proximal tubule rabbit reaggregates receptor removed renal resuspended RPMI scissors Sertoli cells Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells ug/ml vessel viability vitro vivo washed xenobiotics