In Vitro Biological Systems: Methods in Toxicology, Vol. 1, Volume 1Charles A. Tyson, John M. Frazier Methods in Toxicology, Volume 1: In Vitro Biological Systems, Part A provides basic techniques employed by widely recognized scientists to prepare and maintain the biological components of in vitro model systems. The book discusses the in vitro models of neural and neuromuscular systems; ocular system; respiratory system; cardiovascular system; and gastrointestinal system. The text also describes liver slices; liver hepatocytes; other liver cell systems; proximal tubule fragments; kidney cell culture; reproductive and developmental systems; immune system; and skin. Pharmacologists, toxicologists, cell biologists, physiologists, immunotoxicologists, and molecular toxicologists will find the book invaluable. |
From inside the book
Results 1-5 of 67
Page xvii
... metabolism. In this context, they have proved an indispensable resource for investigative toxicology. More recently, the value of in vitro systems as toxicity tests for chemical Safety/hazard evaluation has been recognized and explored ...
... metabolism. In this context, they have proved an indispensable resource for investigative toxicology. More recently, the value of in vitro systems as toxicity tests for chemical Safety/hazard evaluation has been recognized and explored ...
Page xix
... metabolism, and species differences in expressions of toxicity, to mention a few. In the last 10 years, the value of in vitro systems in toxicity testing has been widely recognized and explored. This recent focus on in vitro models, as ...
... metabolism, and species differences in expressions of toxicity, to mention a few. In the last 10 years, the value of in vitro systems in toxicity testing has been widely recognized and explored. This recent focus on in vitro models, as ...
Page 1
... metabolism, and other aspects of neural function. Its laminated structure and diversity of cellular and synaptic ... metabolic substrates. Artificial cerebrospinal fluid (ACSF) composition (in mM): NaCl, 130 KCI, 3.5 METHODS IN ...
... metabolism, and other aspects of neural function. Its laminated structure and diversity of cellular and synaptic ... metabolic substrates. Artificial cerebrospinal fluid (ACSF) composition (in mM): NaCl, 130 KCI, 3.5 METHODS IN ...
Page 43
... metabolic products Of this amount of tissue. Neurochemical Identification of Specified Neurons Care must be taken to choose a marker for the visualization of neurons of a particular neurochemical type that will be unaffected by the ...
... metabolic products Of this amount of tissue. Neurochemical Identification of Specified Neurons Care must be taken to choose a marker for the visualization of neurons of a particular neurochemical type that will be unaffected by the ...
Page 46
... metabolism, or blood–brain barrier penetration. The extracellular milieu can be freely manipulated, and quantitative assessment of cell injury can be performed rapidly. Furthermore, cell cultures are inexpensive, and many cultures can ...
... metabolism, or blood–brain barrier penetration. The extracellular milieu can be freely manipulated, and quantitative assessment of cell injury can be performed rapidly. Furthermore, cell cultures are inexpensive, and many cultures can ...
Contents
1 | |
94 | |
RESPIRATORY SYSTEM | 110 |
CARDIOVASCULAR SYSTEM | 147 |
GASTROINTESTINAL SYSTEM | 182 |
LIVER SLICES | 222 |
LIVER HEPATOCYTES | 231 |
LIVER OTHER CELL SYSTEMS | 279 |
KIDNEY PROXIMAL TUBULE FRAGMENTS | 330 |
KIDNEY CELL CULTURE | 366 |
REPRODUCTIVE AND DEVELOPMENTAL SYSTEMS | 420 |
IMMUNE SYSTEM | 455 |
SKIN | 504 |
Index | 531 |
Other editions - View all
Common terms and phrases
1993 by Academic Academic Press acid activity aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula cell cultures cell suspension cell types cellular centrifuge tube chemical Clara cells coculture collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation enzyme epithelial cells ethanol explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth HBSS hepatocytes HEPES human incubated isolated kidney Kupffer cells laboratory layer lipocytes liver lymphocytes macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurons Pasteur pipette pellet Percoll perfusion petri dish Pharmacol Physiol pipette plastic plates preparation procedure protein proximal tubule rabbit reaggregates receptor removed renal resuspended RPMI scissors Sertoli cells Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells ug/ml vessel viability vitro vivo washed xenobiotics