In Vitro Biological Systems: Methods in Toxicology, Vol. 1, Volume 1Charles A. Tyson, John M. Frazier Methods in Toxicology, Volume 1: In Vitro Biological Systems, Part A provides basic techniques employed by widely recognized scientists to prepare and maintain the biological components of in vitro model systems. The book discusses the in vitro models of neural and neuromuscular systems; ocular system; respiratory system; cardiovascular system; and gastrointestinal system. The text also describes liver slices; liver hepatocytes; other liver cell systems; proximal tubule fragments; kidney cell culture; reproductive and developmental systems; immune system; and skin. Pharmacologists, toxicologists, cell biologists, physiologists, immunotoxicologists, and molecular toxicologists will find the book invaluable. |
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Page 5
... petri dish with a black bottom for improved visual contrast. Small knives can be made by using cyanoacrylic cement to glue a razor blade chip to the tip of a Pasteur pipette. Slice Maintenance For obvious reasons it is desirable to keep ...
... petri dish with a black bottom for improved visual contrast. Small knives can be made by using cyanoacrylic cement to glue a razor blade chip to the tip of a Pasteur pipette. Slice Maintenance For obvious reasons it is desirable to keep ...
Page 10
... dishes are placed in metal culture dish holders (A. H. Thomas). Blank 15 X 100 mm petri dishes or similar petri dishes containing round or square coverslips or quadrant dishes are loaded into stainless steel canisters (A. H. Thomas) ...
... dishes are placed in metal culture dish holders (A. H. Thomas). Blank 15 X 100 mm petri dishes or similar petri dishes containing round or square coverslips or quadrant dishes are loaded into stainless steel canisters (A. H. Thomas) ...
Page 13
... culture dish holders for subsequent dry heat sterilization. Columbia Jars and Corex Centrifuge Bottles Used Columbia jars and centrifuge bottles are soaked overnight in deionized water. After draining and air drying, they are placed ...
... culture dish holders for subsequent dry heat sterilization. Columbia Jars and Corex Centrifuge Bottles Used Columbia jars and centrifuge bottles are soaked overnight in deionized water. After draining and air drying, they are placed ...
Page 14
... petri dish containing sterile distilled water, and the process is repeated, progressing to the proximal part of the tail until very little tendon remains. The tendons which have been collected in water are then teased apart with No. 5 ...
... petri dish containing sterile distilled water, and the process is repeated, progressing to the proximal part of the tail until very little tendon remains. The tendons which have been collected in water are then teased apart with No. 5 ...
Page 15
... culture when applied chronically. Chick embryo extract is prepared by collecting 9-day chick embryos in BSS in a petri dish. The eyes are removed, and the embryos are washed in several changes of BSS. The embryos are then placed in a ...
... culture when applied chronically. Chick embryo extract is prepared by collecting 9-day chick embryos in BSS in a petri dish. The eyes are removed, and the embryos are washed in several changes of BSS. The embryos are then placed in a ...
Contents
1 | |
94 | |
RESPIRATORY SYSTEM | 110 |
CARDIOVASCULAR SYSTEM | 147 |
GASTROINTESTINAL SYSTEM | 182 |
LIVER SLICES | 222 |
LIVER HEPATOCYTES | 231 |
LIVER OTHER CELL SYSTEMS | 279 |
KIDNEY PROXIMAL TUBULE FRAGMENTS | 330 |
KIDNEY CELL CULTURE | 366 |
REPRODUCTIVE AND DEVELOPMENTAL SYSTEMS | 420 |
IMMUNE SYSTEM | 455 |
SKIN | 504 |
Index | 531 |
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Common terms and phrases
1993 by Academic Academic Press acid activity aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula cell cultures cell suspension cell types cellular centrifuge tube chemical Clara cells coculture collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation enzyme epithelial cells ethanol explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth HBSS hepatocytes HEPES human incubated isolated kidney Kupffer cells laboratory layer lipocytes liver lymphocytes macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurons Pasteur pipette pellet Percoll perfusion petri dish Pharmacol Physiol pipette plastic plates preparation procedure protein proximal tubule rabbit reaggregates receptor removed renal resuspended RPMI scissors Sertoli cells Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells ug/ml vessel viability vitro vivo washed xenobiotics