In Vitro Biological Systems: Methods in Toxicology, Vol. 1, Volume 1Charles A. Tyson, John M. Frazier Methods in Toxicology, Volume 1: In Vitro Biological Systems, Part A provides basic techniques employed by widely recognized scientists to prepare and maintain the biological components of in vitro model systems. The book discusses the in vitro models of neural and neuromuscular systems; ocular system; respiratory system; cardiovascular system; and gastrointestinal system. The text also describes liver slices; liver hepatocytes; other liver cell systems; proximal tubule fragments; kidney cell culture; reproductive and developmental systems; immune system; and skin. Pharmacologists, toxicologists, cell biologists, physiologists, immunotoxicologists, and molecular toxicologists will find the book invaluable. |
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Page 5
... pipette (such as a reversed Pasteur pipette). Slices cut by vibratome will consist of hippocampus embedded in other tissue. These slices can be used as is, or the hippocampal portion can be dissected free. This and other microdissection ...
... pipette (such as a reversed Pasteur pipette). Slices cut by vibratome will consist of hippocampus embedded in other tissue. These slices can be used as is, or the hippocampal portion can be dissected free. This and other microdissection ...
Page 9
... pipettes (Bellco, Vineland, NJ) are the mainstay of most of the culture feeding and fluid transferring operations. One other variety of pipette that is used is a 10-ml graduated serological pipette with a large bore tip (Kimax, VWR ...
... pipettes (Bellco, Vineland, NJ) are the mainstay of most of the culture feeding and fluid transferring operations. One other variety of pipette that is used is a 10-ml graduated serological pipette with a large bore tip (Kimax, VWR ...
Page 10
... Pipettes are sterilized in glass pipette canisters (Bellco). Other items are wrapped in a heat-resistant paper (Patapar Steriroll; Paterson Pacific Parchments Co., Sparks, NV) for dry heat sterilization. Items such as rubber stoppers ...
... Pipettes are sterilized in glass pipette canisters (Bellco). Other items are wrapped in a heat-resistant paper (Patapar Steriroll; Paterson Pacific Parchments Co., Sparks, NV) for dry heat sterilization. Items such as rubber stoppers ...
Page 17
... pipette, and the distal 0.5 mm closest to the operator is removed by crosscutting with a new pair of scalpel blades whose tips have been dipped in nutrient medium for lubrication. The cerebellum is then cross cut into seven or eight ...
... pipette, and the distal 0.5 mm closest to the operator is removed by crosscutting with a new pair of scalpel blades whose tips have been dipped in nutrient medium for lubrication. The cerebellum is then cross cut into seven or eight ...
Page 18
... pipettes into nutrient medium, and the remainder of the steps are as described for Cerebellum. Spinal Cord–Dorsal Root ... pipette with nutrient medium, collected in a quadrant dish with medium, and then explanted and incubated as ...
... pipettes into nutrient medium, and the remainder of the steps are as described for Cerebellum. Spinal Cord–Dorsal Root ... pipette with nutrient medium, collected in a quadrant dish with medium, and then explanted and incubated as ...
Contents
1 | |
94 | |
RESPIRATORY SYSTEM | 110 |
CARDIOVASCULAR SYSTEM | 147 |
GASTROINTESTINAL SYSTEM | 182 |
LIVER SLICES | 222 |
LIVER HEPATOCYTES | 231 |
LIVER OTHER CELL SYSTEMS | 279 |
KIDNEY PROXIMAL TUBULE FRAGMENTS | 330 |
KIDNEY CELL CULTURE | 366 |
REPRODUCTIVE AND DEVELOPMENTAL SYSTEMS | 420 |
IMMUNE SYSTEM | 455 |
SKIN | 504 |
Index | 531 |
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Common terms and phrases
1993 by Academic Academic Press acid activity aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula cell cultures cell suspension cell types cellular centrifuge tube chemical Clara cells coculture collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation enzyme epithelial cells ethanol explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth HBSS hepatocytes HEPES human incubated isolated kidney Kupffer cells laboratory layer lipocytes liver lymphocytes macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurons Pasteur pipette pellet Percoll perfusion petri dish Pharmacol Physiol pipette plastic plates preparation procedure protein proximal tubule rabbit reaggregates receptor removed renal resuspended RPMI scissors Sertoli cells Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells ug/ml vessel viability vitro vivo washed xenobiotics