In Vitro Biological Systems: Methods in Toxicology, Vol. 1, Volume 1Charles A. Tyson, John M. Frazier Methods in Toxicology, Volume 1: In Vitro Biological Systems, Part A provides basic techniques employed by widely recognized scientists to prepare and maintain the biological components of in vitro model systems. The book discusses the in vitro models of neural and neuromuscular systems; ocular system; respiratory system; cardiovascular system; and gastrointestinal system. The text also describes liver slices; liver hepatocytes; other liver cell systems; proximal tubule fragments; kidney cell culture; reproductive and developmental systems; immune system; and skin. Pharmacologists, toxicologists, cell biologists, physiologists, immunotoxicologists, and molecular toxicologists will find the book invaluable. |
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Page viii
... Rabbit Kidneys without the Use of Collagenase 330 Klaus Brendel, J. Thomas Hjelle, and Elias Meezan 30. Suspension Culture of Rabbit Renal Proximal Tubules 339 Kathleen G. Dickman and Lazaro J. Mandel 31. Perfusion Method for Isolation ...
... Rabbit Kidneys without the Use of Collagenase 330 Klaus Brendel, J. Thomas Hjelle, and Elias Meezan 30. Suspension Culture of Rabbit Renal Proximal Tubules 339 Kathleen G. Dickman and Lazaro J. Mandel 31. Perfusion Method for Isolation ...
Page 36
... rabbit antibodies against tyrosine hydroxylase (TH) and 5-HT, respectively (13,19). To evaluate more subtle changes in cellular morphology, reaggregate tissue sections can be prepared for ultrastructural analysis (13). Quantitation and ...
... rabbit antibodies against tyrosine hydroxylase (TH) and 5-HT, respectively (13,19). To evaluate more subtle changes in cellular morphology, reaggregate tissue sections can be prepared for ultrastructural analysis (13). Quantitation and ...
Page 65
... rabbit (New Zealand White) is sacrificed by injecting a euthanasia solution (Beuthanasia-D, Schering, Kenilworth, NJ; 0.2 ml/kg body weight) in the marginal ear vein using a 22-gauge needle. The hair overlying the skull is shaved. A ...
... rabbit (New Zealand White) is sacrificed by injecting a euthanasia solution (Beuthanasia-D, Schering, Kenilworth, NJ; 0.2 ml/kg body weight) in the marginal ear vein using a 22-gauge needle. The hair overlying the skull is shaved. A ...
Page 66
... rabbit dorsal root ganglia which includes Percoll gradient separation of myelin debris has been described by Goldenberg and De Boni (9). However, in our hands the inclusion of such a step invariably lowered the yield Of viable neurons ...
... rabbit dorsal root ganglia which includes Percoll gradient separation of myelin debris has been described by Goldenberg and De Boni (9). However, in our hands the inclusion of such a step invariably lowered the yield Of viable neurons ...
Page 94
... rabbit about five. The most superficial of the epithelial cells are decorated on their apical surface with numerous microplicae which serve to anchor the aqueous tear film, thereby establishing the smooth optical surface of the cornea ...
... rabbit about five. The most superficial of the epithelial cells are decorated on their apical surface with numerous microplicae which serve to anchor the aqueous tear film, thereby establishing the smooth optical surface of the cornea ...
Contents
1 | |
94 | |
RESPIRATORY SYSTEM | 110 |
CARDIOVASCULAR SYSTEM | 147 |
GASTROINTESTINAL SYSTEM | 182 |
LIVER SLICES | 222 |
LIVER HEPATOCYTES | 231 |
LIVER OTHER CELL SYSTEMS | 279 |
KIDNEY PROXIMAL TUBULE FRAGMENTS | 330 |
KIDNEY CELL CULTURE | 366 |
REPRODUCTIVE AND DEVELOPMENTAL SYSTEMS | 420 |
IMMUNE SYSTEM | 455 |
SKIN | 504 |
Index | 531 |
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Common terms and phrases
1993 by Academic Academic Press acid activity aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula cell cultures cell suspension cell types cellular centrifuge tube chemical Clara cells coculture collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation enzyme epithelial cells ethanol explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth HBSS hepatocytes HEPES human incubated isolated kidney Kupffer cells laboratory layer lipocytes liver lymphocytes macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurons Pasteur pipette pellet Percoll perfusion petri dish Pharmacol Physiol pipette plastic plates preparation procedure protein proximal tubule rabbit reaggregates receptor removed renal resuspended RPMI scissors Sertoli cells Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells ug/ml vessel viability vitro vivo washed xenobiotics