In Vitro Biological Systems: Methods in Toxicology, Vol. 1, Volume 1Charles A. Tyson, John M. Frazier Methods in Toxicology, Volume 1: In Vitro Biological Systems, Part A provides basic techniques employed by widely recognized scientists to prepare and maintain the biological components of in vitro model systems. The book discusses the in vitro models of neural and neuromuscular systems; ocular system; respiratory system; cardiovascular system; and gastrointestinal system. The text also describes liver slices; liver hepatocytes; other liver cell systems; proximal tubule fragments; kidney cell culture; reproductive and developmental systems; immune system; and skin. Pharmacologists, toxicologists, cell biologists, physiologists, immunotoxicologists, and molecular toxicologists will find the book invaluable. |
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Page 35
... resuspended in 1 ml of water for spectrophotometric determination of protein content using bicinchoninic acid (BCA) (24). Aliquots of the pellet suspension are brought to a volume of 1 ml with water. Standards of bovine serum albumin ...
... resuspended in 1 ml of water for spectrophotometric determination of protein content using bicinchoninic acid (BCA) (24). Aliquots of the pellet suspension are brought to a volume of 1 ml with water. Standards of bovine serum albumin ...
Page 66
... resuspended in 14 ml of basal L-15 medium and the cells washed by centrifugation two more times. Finally, the pellet is resuspended in 6.1 ml of complete L-15 growth medium, and a small sample (80 ul) is taken to determine the yield of ...
... resuspended in 14 ml of basal L-15 medium and the cells washed by centrifugation two more times. Finally, the pellet is resuspended in 6.1 ml of complete L-15 growth medium, and a small sample (80 ul) is taken to determine the yield of ...
Page 84
... resuspended in growth medium at a concentration of 1 x 10° cells per flask. Cells are then plated on plastic culture ware coated with rat tail collagen and allowed to grow to one-half confluency. The medium is then replaced with medium ...
... resuspended in growth medium at a concentration of 1 x 10° cells per flask. Cells are then plated on plastic culture ware coated with rat tail collagen and allowed to grow to one-half confluency. The medium is then replaced with medium ...
Page 87
... resuspended in Krebs-Ringer solution at a density of 2–3 × 10° cells/ml. Cell viability is monitored by trypan blue dye exclusion, and only cell suspensions that exclude dye greater than. 95%. are. used. One-milliliter. samples. (2–3. x. 10 ...
... resuspended in Krebs-Ringer solution at a density of 2–3 × 10° cells/ml. Cell viability is monitored by trypan blue dye exclusion, and only cell suspensions that exclude dye greater than. 95%. are. used. One-milliliter. samples. (2–3. x. 10 ...
Page 88
... resuspended at a cell count of 2.5 x 107 in 2.5 ml of the loading buffer. With gentle stirring, 25 ul of Fura-2/AM stock solution in DMSO is added to the cell suspension to give a final concentration of 10 u/M. The cells are then ...
... resuspended at a cell count of 2.5 x 107 in 2.5 ml of the loading buffer. With gentle stirring, 25 ul of Fura-2/AM stock solution in DMSO is added to the cell suspension to give a final concentration of 10 u/M. The cells are then ...
Contents
1 | |
94 | |
RESPIRATORY SYSTEM | 110 |
CARDIOVASCULAR SYSTEM | 147 |
GASTROINTESTINAL SYSTEM | 182 |
LIVER SLICES | 222 |
LIVER HEPATOCYTES | 231 |
LIVER OTHER CELL SYSTEMS | 279 |
KIDNEY PROXIMAL TUBULE FRAGMENTS | 330 |
KIDNEY CELL CULTURE | 366 |
REPRODUCTIVE AND DEVELOPMENTAL SYSTEMS | 420 |
IMMUNE SYSTEM | 455 |
SKIN | 504 |
Index | 531 |
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Common terms and phrases
1993 by Academic Academic Press acid activity aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula cell cultures cell suspension cell types cellular centrifuge tube chemical Clara cells coculture collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation enzyme epithelial cells ethanol explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth HBSS hepatocytes HEPES human incubated isolated kidney Kupffer cells laboratory layer lipocytes liver lymphocytes macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurons Pasteur pipette pellet Percoll perfusion petri dish Pharmacol Physiol pipette plastic plates preparation procedure protein proximal tubule rabbit reaggregates receptor removed renal resuspended RPMI scissors Sertoli cells Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells ug/ml vessel viability vitro vivo washed xenobiotics