In Vitro Biological Systems: Methods in Toxicology, Vol. 1, Volume 1Charles A. Tyson, John M. Frazier Methods in Toxicology, Volume 1: In Vitro Biological Systems, Part A provides basic techniques employed by widely recognized scientists to prepare and maintain the biological components of in vitro model systems. The book discusses the in vitro models of neural and neuromuscular systems; ocular system; respiratory system; cardiovascular system; and gastrointestinal system. The text also describes liver slices; liver hepatocytes; other liver cell systems; proximal tubule fragments; kidney cell culture; reproductive and developmental systems; immune system; and skin. Pharmacologists, toxicologists, cell biologists, physiologists, immunotoxicologists, and molecular toxicologists will find the book invaluable. |
From inside the book
Results 1-5 of 62
Page ix
... Skin 44. Penetration Studies with Excised Human and Animal Skin Barbara W. Kemppainen 45. Porcine Skin Flaps J. Edmond Riviere and Nancy A. Monteiro-Riviere 46. Keratinocytes Patricia A. Egner and Thomas W. Kensler Index 504 515 525 531 ...
... Skin 44. Penetration Studies with Excised Human and Animal Skin Barbara W. Kemppainen 45. Porcine Skin Flaps J. Edmond Riviere and Nancy A. Monteiro-Riviere 46. Keratinocytes Patricia A. Egner and Thomas W. Kensler Index 504 515 525 531 ...
Page 13
... skin is cut on two sides of the tail with surgical scissors and progressively stripped with the tissue forceps until the tail is free of skin. After transfer to another sterile 20 X 150 mm petri dish, the tail is grasped near the tip ...
... skin is cut on two sides of the tail with surgical scissors and progressively stripped with the tissue forceps until the tail is free of skin. After transfer to another sterile 20 X 150 mm petri dish, the tail is grasped near the tip ...
Page 16
... skin is incised in the midline, beginning over the forehead and extending to the level of the scapulae. With a second scalpel, two incisions perpendicular to the midline incision are made at the rostral end. The skin flaps thereby ...
... skin is incised in the midline, beginning over the forehead and extending to the level of the scapulae. With a second scalpel, two incisions perpendicular to the midline incision are made at the rostral end. The skin flaps thereby ...
Page 17
... skin flaps. Then the mice are not turned, but are left facing away from the operator. The skull is pierced over the posterior fossa, and the midline cut is made in the rostral direction. Skull flaps are similarly made by cutting along ...
... skin flaps. Then the mice are not turned, but are left facing away from the operator. The skull is pierced over the posterior fossa, and the midline cut is made in the rostral direction. Skull flaps are similarly made by cutting along ...
Page 18
... skin and peritoneum are opened, and the entire uterus with contained fetuses is aseptically removed and collected in a petri dish with BSS. After several rinses with BSS, the two horns of the uterus are incised and the fetuses removed ...
... skin and peritoneum are opened, and the entire uterus with contained fetuses is aseptically removed and collected in a petri dish with BSS. After several rinses with BSS, the two horns of the uterus are incised and the fetuses removed ...
Contents
1 | |
94 | |
RESPIRATORY SYSTEM | 110 |
CARDIOVASCULAR SYSTEM | 147 |
GASTROINTESTINAL SYSTEM | 182 |
LIVER SLICES | 222 |
LIVER HEPATOCYTES | 231 |
LIVER OTHER CELL SYSTEMS | 279 |
KIDNEY PROXIMAL TUBULE FRAGMENTS | 330 |
KIDNEY CELL CULTURE | 366 |
REPRODUCTIVE AND DEVELOPMENTAL SYSTEMS | 420 |
IMMUNE SYSTEM | 455 |
SKIN | 504 |
Index | 531 |
Other editions - View all
Common terms and phrases
1993 by Academic Academic Press acid activity aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula cell cultures cell suspension cell types cellular centrifuge tube chemical Clara cells coculture collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation enzyme epithelial cells ethanol explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth HBSS hepatocytes HEPES human incubated isolated kidney Kupffer cells laboratory layer lipocytes liver lymphocytes macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurons Pasteur pipette pellet Percoll perfusion petri dish Pharmacol Physiol pipette plastic plates preparation procedure protein proximal tubule rabbit reaggregates receptor removed renal resuspended RPMI scissors Sertoli cells Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells ug/ml vessel viability vitro vivo washed xenobiotics