In Vitro Biological Systems: Methods in Toxicology, Vol. 1, Volume 1Charles A. Tyson, John M. Frazier Methods in Toxicology, Volume 1: In Vitro Biological Systems, Part A provides basic techniques employed by widely recognized scientists to prepare and maintain the biological components of in vitro model systems. The book discusses the in vitro models of neural and neuromuscular systems; ocular system; respiratory system; cardiovascular system; and gastrointestinal system. The text also describes liver slices; liver hepatocytes; other liver cell systems; proximal tubule fragments; kidney cell culture; reproductive and developmental systems; immune system; and skin. Pharmacologists, toxicologists, cell biologists, physiologists, immunotoxicologists, and molecular toxicologists will find the book invaluable. |
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Page 9
... sodium metasilicate crystals, and 3.5 ounce bars of Ivory soap. The sodium metasilicate is maintained as a stock solution of 200 g crystals dissolved in 1 liter of deionized water by heating. The Ivory soap is kept in solution by adding ...
... sodium metasilicate crystals, and 3.5 ounce bars of Ivory soap. The sodium metasilicate is maintained as a stock solution of 200 g crystals dissolved in 1 liter of deionized water by heating. The Ivory soap is kept in solution by adding ...
Page 12
... sodium metasilicate solution. The mixture is brought to a boil, and boiling is continued gently for 10 min. The glassware is then transferred to another tank containing simmering deionized water. This tank is then removed from the heat ...
... sodium metasilicate solution. The mixture is brought to a boil, and boiling is continued gently for 10 min. The glassware is then transferred to another tank containing simmering deionized water. This tank is then removed from the heat ...
Page 29
... sodium bicarbonate. Stir until dissolved. Filter the medium under reduced pressure into sterile 500-ml bottles using a filter with a 0.2-um membrane (i.e., Costar, Cambridge, MA, bottle-top filter, Cat. No. 8330). The sterile medium is ...
... sodium bicarbonate. Stir until dissolved. Filter the medium under reduced pressure into sterile 500-ml bottles using a filter with a 0.2-um membrane (i.e., Costar, Cambridge, MA, bottle-top filter, Cat. No. 8330). The sterile medium is ...
Page 30
... sodium phosphate-buffered saline (pH 7.4). Preparation of Medium for Culturing Reaggregates Medium is prepared fresh before use and consists of the following: 100 ml BME, 10 ml dialyzed fetal bovine or horse serum, 2.5 ml DNase solution ...
... sodium phosphate-buffered saline (pH 7.4). Preparation of Medium for Culturing Reaggregates Medium is prepared fresh before use and consists of the following: 100 ml BME, 10 ml dialyzed fetal bovine or horse serum, 2.5 ml DNase solution ...
Page 35
... sodium borate (decahydrate, pH 9.2). The derivatization proceeds for 1 min before injection of 20 ul into the HPLC system. The HPLC system used for determination of GABA levels is similar to that described in Kontur et al. (23) with ...
... sodium borate (decahydrate, pH 9.2). The derivatization proceeds for 1 min before injection of 20 ul into the HPLC system. The HPLC system used for determination of GABA levels is similar to that described in Kontur et al. (23) with ...
Contents
1 | |
94 | |
RESPIRATORY SYSTEM | 110 |
CARDIOVASCULAR SYSTEM | 147 |
GASTROINTESTINAL SYSTEM | 182 |
LIVER SLICES | 222 |
LIVER HEPATOCYTES | 231 |
LIVER OTHER CELL SYSTEMS | 279 |
KIDNEY PROXIMAL TUBULE FRAGMENTS | 330 |
KIDNEY CELL CULTURE | 366 |
REPRODUCTIVE AND DEVELOPMENTAL SYSTEMS | 420 |
IMMUNE SYSTEM | 455 |
SKIN | 504 |
Index | 531 |
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1993 by Academic Academic Press acid activity aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula cell cultures cell suspension cell types cellular centrifuge tube chemical Clara cells coculture collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation enzyme epithelial cells ethanol explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth HBSS hepatocytes HEPES human incubated isolated kidney Kupffer cells laboratory layer lipocytes liver lymphocytes macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurons Pasteur pipette pellet Percoll perfusion petri dish Pharmacol Physiol pipette plastic plates preparation procedure protein proximal tubule rabbit reaggregates receptor removed renal resuspended RPMI scissors Sertoli cells Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells ug/ml vessel viability vitro vivo washed xenobiotics