In Vitro Biological Systems: Methods in Toxicology, Vol. 1, Volume 1Charles A. Tyson, John M. Frazier Methods in Toxicology, Volume 1: In Vitro Biological Systems, Part A provides basic techniques employed by widely recognized scientists to prepare and maintain the biological components of in vitro model systems. The book discusses the in vitro models of neural and neuromuscular systems; ocular system; respiratory system; cardiovascular system; and gastrointestinal system. The text also describes liver slices; liver hepatocytes; other liver cell systems; proximal tubule fragments; kidney cell culture; reproductive and developmental systems; immune system; and skin. Pharmacologists, toxicologists, cell biologists, physiologists, immunotoxicologists, and molecular toxicologists will find the book invaluable. |
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Page 10
... sterile room in which all culture setup and feeding procedures are performed is equipped with horizontal laminar flow hoods. Entrance to the sterile room is permitted only by operators wearing disposable paper surgical caps, masks, and ...
... sterile room in which all culture setup and feeding procedures are performed is equipped with horizontal laminar flow hoods. Entrance to the sterile room is permitted only by operators wearing disposable paper surgical caps, masks, and ...
Page 13
... sterile 20 x 150 mm Petri dish, the most proximal vertebra is removed by cutting through the nearest joint with a scalpel and discarded. The base of the tail is grasped with a pair of straight Rochester-Paen hemostatic forceps, and the ...
... sterile 20 x 150 mm Petri dish, the most proximal vertebra is removed by cutting through the nearest joint with a scalpel and discarded. The base of the tail is grasped with a pair of straight Rochester-Paen hemostatic forceps, and the ...
Page 14
... sterile distilled water, and the process is repeated, progressing to the proximal part of the tail until very little tendon remains. The tendons which have been collected in water are then teased apart with No. 5 INOX forceps and ...
... sterile distilled water, and the process is repeated, progressing to the proximal part of the tail until very little tendon remains. The tendons which have been collected in water are then teased apart with No. 5 INOX forceps and ...
Page 15
... sterile, and antibiotics are not added. In most instances, the media used during explantation are the same as those ... sterile test tube without cord stripping, which causes hemolysis. The collected blood is allowed to clot for an hour ...
... sterile, and antibiotics are not added. In most instances, the media used during explantation are the same as those ... sterile test tube without cord stripping, which causes hemolysis. The collected blood is allowed to clot for an hour ...
Page 17
... sterile 20 X 150 mm petri dish to prevent drying of the collagen or the culture. Lining the petri dish with black filter paper (Whatman; A. H. Thomas) improves visibility of the explant. Each Maximow slide is dubbed with sterile ...
... sterile 20 X 150 mm petri dish to prevent drying of the collagen or the culture. Lining the petri dish with black filter paper (Whatman; A. H. Thomas) improves visibility of the explant. Each Maximow slide is dubbed with sterile ...
Contents
1 | |
94 | |
RESPIRATORY SYSTEM | 110 |
CARDIOVASCULAR SYSTEM | 147 |
GASTROINTESTINAL SYSTEM | 182 |
LIVER SLICES | 222 |
LIVER HEPATOCYTES | 231 |
LIVER OTHER CELL SYSTEMS | 279 |
KIDNEY PROXIMAL TUBULE FRAGMENTS | 330 |
KIDNEY CELL CULTURE | 366 |
REPRODUCTIVE AND DEVELOPMENTAL SYSTEMS | 420 |
IMMUNE SYSTEM | 455 |
SKIN | 504 |
Index | 531 |
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Common terms and phrases
1993 by Academic Academic Press acid activity aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula cell cultures cell suspension cell types cellular centrifuge tube chemical Clara cells coculture collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation enzyme epithelial cells ethanol explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth HBSS hepatocytes HEPES human incubated isolated kidney Kupffer cells laboratory layer lipocytes liver lymphocytes macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurons Pasteur pipette pellet Percoll perfusion petri dish Pharmacol Physiol pipette plastic plates preparation procedure protein proximal tubule rabbit reaggregates receptor removed renal resuspended RPMI scissors Sertoli cells Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells ug/ml vessel viability vitro vivo washed xenobiotics