In Vitro Biological Systems: Methods in Toxicology, Vol. 1, Volume 1Charles A. Tyson, John M. Frazier Methods in Toxicology, Volume 1: In Vitro Biological Systems, Part A provides basic techniques employed by widely recognized scientists to prepare and maintain the biological components of in vitro model systems. The book discusses the in vitro models of neural and neuromuscular systems; ocular system; respiratory system; cardiovascular system; and gastrointestinal system. The text also describes liver slices; liver hepatocytes; other liver cell systems; proximal tubule fragments; kidney cell culture; reproductive and developmental systems; immune system; and skin. Pharmacologists, toxicologists, cell biologists, physiologists, immunotoxicologists, and molecular toxicologists will find the book invaluable. |
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Page 31
... trypsin (GIBCO, Cat. No. 610-5095) for 35 min at 37°C. The trypsin-containing Solution is aspirated with a Pasteur pipette at the end of this period and remainA RHOMBIC LIP CEREBRAL LOBE MEDULLA MESENCEPHALIC FLEXURE B TECTUM C 3 ...
... trypsin (GIBCO, Cat. No. 610-5095) for 35 min at 37°C. The trypsin-containing Solution is aspirated with a Pasteur pipette at the end of this period and remainA RHOMBIC LIP CEREBRAL LOBE MEDULLA MESENCEPHALIC FLEXURE B TECTUM C 3 ...
Page 47
... Trypsin (0.25%) 0.25 g trypsin (Sigma, St. Louis, MO, T-6763) 100 ml 1X dissecting medium Filter, freeze in aliquots at −70°C for up to 2 months. Trypsin is diluted to 0.09% with media Stock before use. Glutamine (200 mM) 100X ...
... Trypsin (0.25%) 0.25 g trypsin (Sigma, St. Louis, MO, T-6763) 100 ml 1X dissecting medium Filter, freeze in aliquots at −70°C for up to 2 months. Trypsin is diluted to 0.09% with media Stock before use. Glutamine (200 mM) 100X ...
Page 49
... trypsin to the tube of MS, divide the 4 ml of solution equally into two 15-ml centrifuge tubes, and place them on ice. 3. Fill two 60-mm culture dishes with 70% ethanol and Set them on the dissecting table. Sterilize the following ...
... trypsin to the tube of MS, divide the 4 ml of solution equally into two 15-ml centrifuge tubes, and place them on ice. 3. Fill two 60-mm culture dishes with 70% ethanol and Set them on the dissecting table. Sterilize the following ...
Page 51
... trypsin solution (two 15-ml centrifuge tubes) with a pipette. Incubate the tissue for 1 hr in a 37°C water bath. Plating Mixed Cortical Neuronal and Glial Cells 1. Remove the cells from the water bath and place them in a clinical ...
... trypsin solution (two 15-ml centrifuge tubes) with a pipette. Incubate the tissue for 1 hr in a 37°C water bath. Plating Mixed Cortical Neuronal and Glial Cells 1. Remove the cells from the water bath and place them in a clinical ...
Page 62
... Trypsin: Dissolve 100 mg trypsin (type III, Sigma T-8253) in 500 ul Ringer's solution; prepare 25-pul aliquots and store at -20°C. Collagenase: Dissolve 50 mg collagenase (type IIS, Sigma C-1764) in 500 ul of Ringer's solution; prepare ...
... Trypsin: Dissolve 100 mg trypsin (type III, Sigma T-8253) in 500 ul Ringer's solution; prepare 25-pul aliquots and store at -20°C. Collagenase: Dissolve 50 mg collagenase (type IIS, Sigma C-1764) in 500 ul of Ringer's solution; prepare ...
Contents
1 | |
94 | |
RESPIRATORY SYSTEM | 110 |
CARDIOVASCULAR SYSTEM | 147 |
GASTROINTESTINAL SYSTEM | 182 |
LIVER SLICES | 222 |
LIVER HEPATOCYTES | 231 |
LIVER OTHER CELL SYSTEMS | 279 |
KIDNEY PROXIMAL TUBULE FRAGMENTS | 330 |
KIDNEY CELL CULTURE | 366 |
REPRODUCTIVE AND DEVELOPMENTAL SYSTEMS | 420 |
IMMUNE SYSTEM | 455 |
SKIN | 504 |
Index | 531 |
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Common terms and phrases
1993 by Academic Academic Press acid activity aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula cell cultures cell suspension cell types cellular centrifuge tube chemical Clara cells coculture collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation enzyme epithelial cells ethanol explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth HBSS hepatocytes HEPES human incubated isolated kidney Kupffer cells laboratory layer lipocytes liver lymphocytes macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurons Pasteur pipette pellet Percoll perfusion petri dish Pharmacol Physiol pipette plastic plates preparation procedure protein proximal tubule rabbit reaggregates receptor removed renal resuspended RPMI scissors Sertoli cells Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells ug/ml vessel viability vitro vivo washed xenobiotics