Electrophoretic and Isoelectric Focusing Techniques in Fisheries Management
Probably the most ubiquitous biochemical method used today for examining the genetics of individuals, populations, or phylogenetic relationships between taxa is electrophoresis. This book has been created to offer a viewpoint regarding current electrophoretic separation methodologies of macromolecules and their major applications to fisheries management. The chapters in this book have been selected and organized into three sections to create a carefully blended mixture of methodologies and applications designed to educate the novice, as well as stimulate interest in professional researchers currently using electrophoresis for their work. The first section includes chapters that discuss the principles that explain the genetic basis of multiple molecular forms of proteins, the theory and practice of DNA analyses, and the methodology of electrophoretic separation of these macromolecules; starch gel electrophoresis as the predominant electrophoretic tool for fisheries genetics; and protein isoelectric focusing and DNA analysis. The second section describes a variety of applications for electrophoretic techniques. The third section presents a discussion and results of experiments conducted by Dennis Powers and his associates regarding the physiological significance of multiple forms of enzymes using the fish Fundulus heteroclitus as a model system. The book features a catalog of nearly 100 enzyme staining recipes and covers new areas in electrophoretic work, such as DNA fingerprinting, genetic tags, mitochondrial DNA methodologies, and genomic manipulation of fish stocks. This book will provide a useful reference resource for fisheries biologists at federal, state, and local levels; fisheries researchers at universities; and students pursuing degrees involving research in fish genetics.
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Interpretation of Electrophoretic Data
Starch Gel Electrophoresis and Histochemical Visualization of Proteins
The Integration of Allozyme Analyses and Genomic Manipulations
acid agar agarose allele frequencies Allendorf allozyme Alternate name(s ampholyte analysis application Aquaculture Aquat Atlantic salmon Avise bands Biochem Biol brown trout buffer chromosome chum salmon dehydrogenase detected developmental differentiation dimeric diploid eggs electrode electrophoretic enzyme extracts Figure fish Fishery Management fragments gel slice gene expression gene products genetic marking genetic variation genomic genotypes gradient gynogenesis haplotypes hatchery heterozygotes heterozygous Homozygote homozygous identified Incubate at 37°C individuals interspecific introgression invasive tissue sampling isoelectric focusing isolation isozyme largemouth bass LDH-B linkage liver loci locus products markers maternal methods minimally invasive tissue mitochondrial DNA ml Incubate molecular Morizot mtDNA mtDNA haplotypes muscle Oncorhynchus parvalbumins patterns phenotypes poeciliid polymorphism Population Genetics population structure probes procedures progeny protein rainbow trout restriction salmonid Seeb sequence Shaklee Siciliano and Shaw spawning species starch gel electrophoresis Subunit structure sunfish tags techniques temperature triploid Tris/HCl pH 8.0 variability visualization Whitt
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Molecular Genetic Analysis of Populations: A Practical Approach
A. Rus Hoelzel
No preview available - 1998