Electrophoretic and Isoelectric Focusing Techniques in Fisheries ManagementProbably the most ubiquitous biochemical method used today for examining the genetics of individuals, populations, or phylogenetic relationships between taxa is electrophoresis. This book has been created to offer a viewpoint regarding current electrophoretic separation methodologies of macromolecules and their major applications to fisheries management. The chapters in this book have been selected and organized into three sections to create a carefully blended mixture of methodologies and applications designed to educate the novice, as well as stimulate interest in professional researchers currently using electrophoresis for their work. The first section includes chapters that discuss the principles that explain the genetic basis of multiple molecular forms of proteins, the theory and practice of DNA analyses, and the methodology of electrophoretic separation of these macromolecules; starch gel electrophoresis as the predominant electrophoretic tool for fisheries genetics; and protein isoelectric focusing and DNA analysis. The second section describes a variety of applications for electrophoretic techniques. The third section presents a discussion and results of experiments conducted by Dennis Powers and his associates regarding the physiological significance of multiple forms of enzymes using the fish Fundulus heteroclitus as a model system. The book features a catalog of nearly 100 enzyme staining recipes and covers new areas in electrophoretic work, such as DNA fingerprinting, genetic tags, mitochondrial DNA methodologies, and genomic manipulation of fish stocks. This book will provide a useful reference resource for fisheries biologists at federal, state, and local levels; fisheries researchers at universities; and students pursuing degrees involving research in fish genetics. |
From inside the book
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... Variation in Natural Populations Dennis A. Powers Index .... 265 ........ .281 297 ............... 313 ... GENETIC PRINCIPLES AND THE INTERPRETATION OF ELECTROPHORETIC DATA.
... Variation in Natural Populations Dennis A. Powers Index .... 265 ........ .281 297 ............... 313 ... GENETIC PRINCIPLES AND THE INTERPRETATION OF ELECTROPHORETIC DATA.
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... Genetic Principles ..... II . A. Detectable Gene Products .......... 1. Temporal Variation / Ontogeny ........... Spatial Variation / Gene Expression 2 . a . b . Intracellular Limitations ...... Tissue Specificity Structure of Gene ...
... Genetic Principles ..... II . A. Detectable Gene Products .......... 1. Temporal Variation / Ontogeny ........... Spatial Variation / Gene Expression 2 . a . b . Intracellular Limitations ...... Tissue Specificity Structure of Gene ...
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Contents
Chapter | 2 |
Future Work | 19 |
Starch Gel Electrophoresis and Histochemical Visualization of Proteins | 23 |
Chapter 3 | 81 |
Chapter 4 | 107 |
Chapter 5 | 131 |
Chapter 12 | 140 |
Chapter 7 | 157 |
Chapter 9 | 197 |
Chapter 10 | 223 |
Chapter 11 | 241 |
The Integration of Allozyme Analyses and Genomic Manipulations | 265 |
Chapter 13 | 281 |
Chapter 14 | 297 |
Chapter 15 | 313 |
341 | |
Common terms and phrases
0.2 M Tris/HCl acid activity agar agarose allele frequencies Allendorf allozyme ampholyte analysis application Aquaculture Aquat Atlantic salmon Avise Biochem brown trout buffer chromosome chum salmon dehydrogenase detected developmental differentiation dimeric diploid DNA fingerprints eggs electrode electrophoretic enzyme estimates Figure fisheries management fragments gel slice gene expression gene products genetic marking genetic variation genomic genotypes gradient gynogenesis H₂O haplotypes hatchery heterozygosity heterozygous hybrids identified Incubate at 37°C individuals invasive tissue sampling isoelectric focusing isolated isozyme largemouth bass LDH-B linkage liver loci locus products markers methods minimally invasive tissue mitochondrial DNA molecular Morizot mtDNA mtDNA haplotypes muscle NT NT NT parvalbumins patterns phenotypes poeciliid polymorphism Population Genetics population structure procedures progeny protein rainbow trout restriction salmonid Seeb sequence Shaklee Siciliano spawning starch gel electrophoresis stock identification Subunit structure sunfish tags techniques temperature triploid Tris/HCl pH 8.0 variability visualization Whitt
References to this book
Molecular Genetic Analysis of Populations: A Practical Approach A. Rus Hoelzel No preview available - 1998 |