Genetic Manipulation of the Nervous System
Elsevier, Nov 24, 1995 - Science - 276 pages
Neuroscience Perspectives provides multidisciplinary reviews of topics in one of the most diverse and rapidly advancing fields in the life sciences.
Whether you are a new recruit to neuroscience, or an established expert, look to this series for 'one-stop' sources of the historical, physiological, pharmacological, biochemical, molecular biological and therapeutic aspects of chosen research areas.
The recent development of Gene Therapy procedures which allow specific genes to be delivered to human patients who lack functional copies of them is of major therapeutic importance. In addition such gene delivery methods can be used in other organisms to define the function of particular genes. These studies are of particular interest in the nervous system where there are many incurable diseases like Alzheimer's and Parkinson's diseases which may benefit from therapies of this kind. Unfortunately gene delivery methods for use in the nervous system have lagged behind those in other systems due to the fact that the methods developed in other systems are often not applicable to cells like neurons which do not divide. This book discusses a wide range of methods which have now been developed to overcome these problems and allow safe and efficient delivery of particular genes to the brain. Methods discussed include virological methods, physical methods (such as liposomes) and the transplantation of genetically modified cells. In a single volume therefore this book provides a complete view of these methods and indicates how they can be applied to the development of therapies for treating previously incurable neurological disorders.
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a new tool to transfer genes into the central nervous system for treatment o f neurodegenerative disorders
Chapter 4 Adenovirusmediated gene therapy of tumors in the central nervous system
Chapter 5 Gene delivery using adenoassociated virus
Chapter 6 Herpes simplex virusbased vectors
Chapter 7 Gene delivery using herpes simplex virus type 1 plasmid vectors
Chapter 8 Problems in the use of herpes simplex virus as a vector
Chapter 10 Gene delivery to the nervous system using retroviral vectors
Chapter 11 Transplantation of genetically modified nonneuronal cells in the central nervous system
Chapter 12 Genetic animal models for Alzheimers disease
biology and applications
Chapter 14 Direct injection of plasmid DNA into the brain
Chapter 15 Antlsense oligodeoxynucleotides as novel neuropharmacological tools for selective expression blockade in the brain
Color Plate Section
Chapter 9 Gene delivery to the nervous system by direct injection of retroviral vector
Acad activity adeno-associated virus adenovirus adenovirus vectors adenovirus-mediated ADV/RSV-tk Alzheimer's disease antisense AS-ODNs astrocytes Biol brain tumors Breakefield cell lines cell types cellular central nervous system Cepko cloned culture defective deletion differentiation dopamine effects efficiency encoding fibroblasts fl-galactosidase function Gage Geller gene delivery gene expression Gene Ther gene therapy gene transfer genetically modified genome glial cells glioma grafted herpes simplex virus HSV-1 vectors HSV-tk HSV1 human ICP4 implantation infection inhibition lacz latent lesion mammalian migration mouse mRNA mutant Natl nerve growth factor neuronal cells neurons Neurosci NGF-producing ODNs Parkinson's disease Perricaudet plasmid plasmid DNA primary fibroblasts Proc promoter protein rat brain receptor region replication retroviral vectors retrovirus sequences specific striatum studies survival SVZ cells target therapeutic tion tissue toxicity transcription transduction transfected transgene transgene expression transgenic mice transplantation tumor cells tyrosine hydroxylase viral Virol virus vectors viruses vitro vivo gene wild-type