Genetic Manipulation of the Nervous SystemNeuroscience Perspectives provides multidisciplinary reviews of topics in one of the most diverse and rapidly advancing fields in the life sciences. Whether you are a new recruit to neuroscience, or an established expert, look to this series for 'one-stop' sources of the historical, physiological, pharmacological, biochemical, molecular biological and therapeutic aspects of chosen research areas. The recent development of Gene Therapy procedures which allow specific genes to be delivered to human patients who lack functional copies of them is of major therapeutic importance. In addition such gene delivery methods can be used in other organisms to define the function of particular genes. These studies are of particular interest in the nervous system where there are many incurable diseases like Alzheimer's and Parkinson's diseases which may benefit from therapies of this kind. Unfortunately gene delivery methods for use in the nervous system have lagged behind those in other systems due to the fact that the methods developed in other systems are often not applicable to cells like neurons which do not divide. This book discusses a wide range of methods which have now been developed to overcome these problems and allow safe and efficient delivery of particular genes to the brain. Methods discussed include virological methods, physical methods (such as liposomes) and the transplantation of genetically modified cells. In a single volume therefore this book provides a complete view of these methods and indicates how they can be applied to the development of therapies for treating previously incurable neurological disorders. |
Contents
| 1 | |
| 11 | |
| 41 | |
Chapter 4 Adenovirusmediated gene therapy of tumors in the central nervous system | 53 |
Chapter 5 Gene delivery using adenoassociated virus | 73 |
Chapter 6 Herpes simplex virusbased vectors | 99 |
Chapter 7 Gene delivery using herpes simplex virus type 1 plasmid vectors | 115 |
Chapter 8 Problems in the use of herpes simplex virus as a vector | 127 |
Chapter 10 Gene delivery to the nervous system using retroviral vectors | 149 |
Chapter 11 Transplantation of genetically modified nonneuronal cells in the central nervous system | 181 |
Chapter 12 Genetic animal models for Alzheimers disease | 203 |
biology and applications | 221 |
Chapter 14 Direct injection of plasmid DNA into the brain | 235 |
Chapter 15 Antlsense oligodeoxynucleotides as novel neuropharmacological tools for selective expression blockade in the brain | 249 |
Index | 269 |
Color Plate Section | 277 |
Common terms and phrases
Acad activity adeno-associated virus adenovirus adenovirus vectors adenovirus-mediated ADV/RSV-tk Alzheimer's disease antisense AS-ODNs astrocytes Biol brain tumors Breakefield cell lines cell types cellular central nervous system Cepko cloned culture defective deletion differentiation dopamine effects efficiency encoding fibroblasts fl-galactosidase function Gage Geller gene delivery gene expression Gene Ther gene therapy gene transfer genetically modified genome glial cells glioma grafted herpes simplex virus HSV-1 vectors HSV-tk HSV1 human ICP4 implantation infection inhibition lacz latent lesion mammalian migration mouse mRNA mutant Natl nerve growth factor neuronal cells neurons Neurosci NGF-producing ODNs Parkinson's disease Perricaudet plasmid plasmid DNA primary fibroblasts Proc promoter protein rat brain receptor region replication retroviral vectors retrovirus sequences specific striatum studies survival SVZ cells target therapeutic tion tissue toxicity transcription transduction transfected transgene transgene expression transgenic mice transplantation tumor cells tyrosine hydroxylase viral Virol virus vectors viruses vitro vivo gene wild-type
Popular passages
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