Ancient DNA: Recovery and Analysis of Genetic Material from Paleontological, Archaeological, Museum, Medical, and Forensic Specimens

Front Cover
Bernd Herrmann, Susanne Hummel
Springer Science & Business Media, Jun 24, 1994 - Gardening - 263 pages
Ancient DNA refers to DNA which can be recovered and analyzed from clinical, museum, archaeological and paleontological specimens. Ancient DNA ranges in age from less than 100 years to tens of millions of years. The study of ancient DNA is a young field, but it has been revolutionized by the application of polymerase chain reaction technology, and interest is growing very rapidly. Fields as diverse as evolution, anthropology, medicine, agriculture, and even law enforcement have quickly found applications in the recovery of ancient DNA. This book contains contributions from many of the "first generation" researchers who pioneered the development and application of ancient DNA methods. Their chapters present the protocols and precautions which have resulted in the remarkable results obtained in recent years. The range of subjects reflects the wide diversity of applications that are emerging in research on ancient DNA, including the study of DNA to analyze kinship, recovery of DNA from organisms trapped in amber, ancient DNA from human remains preserved in a variety of locations and conditions, DNA recovered from herbarium and museum specimens, and DNA isolated from ancient plant seeds or compression fossils. Ancient DNA will serve as a valuable source of information, ideas, and protocols for anyone interested in this extraordinary field.

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Contents

Introduction
1
2 Simple Repeat Loci as Tools for Genetic Identification
13
3 Spatial and Temporal Aspects of Populations Revealed by Mitochondrial DNA
31
4 General Aspects of Sample Preparation
59
5 Screening for Pathogenic DNA Sequences in Clinically Collected Human Tissues
69
6 DNA from Amber Inclusions
92
7 DNA Analysis of the Windover Population
104
8 DNA from Arctic Human Burials
122
Soft Tissues 11 DNA from Herbarium Specimens
166
Soft Tissues 12 HighFidelity Amplification of Ribosomal Gene Sequences from South American Mummies
182
Hard Tissues 13 Mitochondrial DNA from Ancient Bones
195
Hard Tissues 14 YChromosomal DNA from Ancient Bones
205
Hard Tissues 15 Genomic DNA from Museum Bird Feathers
211
Hard Tissues 16 DNA and RNA from Ancient Plant Seeds
218
17 DNA from Plant Compression Fossils
237
Index
257

Body Fluids 9 DNA Typing of Biological Evidence Material
141
Soft Tissues 10 DNA from Museum Specimens
149

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