Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 326
... bound relaxation rates of —CH , protons of p - acetamidocholine phenyl ether , calculated for the rapid exchange model . The anomalous part of the curve for the N - CH , protons is the dashed line ( Metcalfe et al . , 1968 ) . through a ...
... bound relaxation rates of —CH , protons of p - acetamidocholine phenyl ether , calculated for the rapid exchange model . The anomalous part of the curve for the N - CH , protons is the dashed line ( Metcalfe et al . , 1968 ) . through a ...
Page 328
... bound to the antibody in 1 M NaCl and chloride ions may experience three kinds of environment : ( 1 ) symmetrically solvated by water ; ( 2 ) chloride com- plexed to mercury - labeled hapten which is not bound to antibody ; and ( 3 ) ...
... bound to the antibody in 1 M NaCl and chloride ions may experience three kinds of environment : ( 1 ) symmetrically solvated by water ; ( 2 ) chloride com- plexed to mercury - labeled hapten which is not bound to antibody ; and ( 3 ) ...
Page 370
... bound to the protein . For one or more equivalents of a poorly binding metal ion to be bound it is usual for the concentration of unbound ion to exceed by far that of the protein , so that only a small fraction of the total metal ion is ...
... bound to the protein . For one or more equivalents of a poorly binding metal ion to be bound it is usual for the concentration of unbound ion to exceed by far that of the protein , so that only a small fraction of the total metal ion is ...
Contents
Ultracentrifugal Analysis J H Coates Glossary of Symbols 235 | 3 |
Fundamentals of the Method | 5 |
Light Scattering | 12 |
Copyright | |
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absorption acid amino anions atoms axial ratio axis band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone