Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 340
... imidazole peaks of the four histidine residues . Peak 5 is a C - 4 imidazole resonance . The envelope labeled " aromatic " includes three other C - 4 imidazole peaks as well as peaks from six tyrosine and three phenylalanine residues ...
... imidazole peaks of the four histidine residues . Peak 5 is a C - 4 imidazole resonance . The envelope labeled " aromatic " includes three other C - 4 imidazole peaks as well as peaks from six tyrosine and three phenylalanine residues ...
Page 377
... imidazole groups of the histidyl residues . In contrast with almost all of the other normally charge - bearing groups in a protein , the imidazole groups have intrinsic pKa values close enough to the physio- logical pH range that they ...
... imidazole groups of the histidyl residues . In contrast with almost all of the other normally charge - bearing groups in a protein , the imidazole groups have intrinsic pKa values close enough to the physio- logical pH range that they ...
Page 383
... imidazole groups in that protein . It is more realistic to consider a variation in individual pKa values with the progress of titration of the imidazole groups than to accept the full weight of the electrostatic term as such ...
... imidazole groups in that protein . It is more realistic to consider a variation in individual pKa values with the progress of titration of the imidazole groups than to accept the full weight of the electrostatic term as such ...
Contents
Ultracentrifugal Analysis J H Coates Glossary of Symbols 235 | 3 |
Fundamentals of the Method | 5 |
Light Scattering | 12 |
Copyright | |
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absorption acid amino anions atoms axial ratio axis band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone