Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 220
... optical density and it is desirable to record all spectra which are to be retained for study directly as linear optical density against linear frequency . Both the PE 621 and IR 12 are equipped with this facility . The operation in ...
... optical density and it is desirable to record all spectra which are to be retained for study directly as linear optical density against linear frequency . Both the PE 621 and IR 12 are equipped with this facility . The operation in ...
Page 228
... Optical density for homogeneous dispersion FIG . 6. The effect of a nonuniform distribution of absorbing material in the optical path on the measured optical density ( after Jones , 1952 ) ; x is the fraction of the beam obscured by the ...
... Optical density for homogeneous dispersion FIG . 6. The effect of a nonuniform distribution of absorbing material in the optical path on the measured optical density ( after Jones , 1952 ) ; x is the fraction of the beam obscured by the ...
Page 236
... optical parameters are clearly defined and can be accurately reproduced . Appreciable changes in noise level will occur , and if this is excessive in any particular region it must be rerun with an increased slit setting ( if not already ...
... optical parameters are clearly defined and can be accurately reproduced . Appreciable changes in noise level will occur , and if this is excessive in any particular region it must be rerun with an increased slit setting ( if not already ...
Contents
Ultracentrifugal Analysis J H Coates Glossary of Symbols 235 | 3 |
Fundamentals of the Method | 5 |
Light Scattering | 12 |
Copyright | |
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absorption acid amino anions atoms axial ratio axis band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone