Molecular Cloning: A Laboratory Manual, Volume 1The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity. |
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Results 1-5 of 16
... binding proteins have been partially supplanted by artificial tags that have no counterpart in the natural world . The best known of these are ( 1 ) a polyhistidine sequence that binds to columns carrying Zn2 + or Ni2 + ( Smith et al ...
... binding protein cross - linked amylose maltose ( 10 mM ) Chloramphenicol p - aminochloram- chloramphenicol ( 5 mM ) acetyltransferase phenicol - Sepharose Carbonic anhydrase II sulfonamide affinity Tris - SO4 ( pH 6.8 ) METHOD OF ...
... binding site is important for the expression of cloned DNA in E. coli . The ribosome - binding site includes an initiation codon ( ATG ) and a sequence 5-9 nucleotides in length located 3-11 nucleotides upstream of the initiation codon ...
... BINDING PROTEIN ( MBP ) FUSIONS GENERATE RECOMBINANT PROTEINS in a form that can be purified to near homogeneity by ... bind to the affinity resin . The Factor X protease cleavage site ( Ile - Glu - Gly - Arg ) lies immediately adjacent ...
... BIND TIGHTLY TO A NUMBER OF TRANSITION METALS and transition metal chelate complexes , such that a protein carrying an ... binding partner can allow isolation of the binary complex on the affinity matrix and selec- tive elution of the ...
Contents
15-3 | |
15-12 | |
15-49 | |
15-55 | |
15-64 | |
16-6 | |
16-7 | |
16-12 | |
Chapter 12 | 12 |
Chapter 9 | 11 |
1 | 13 |
In Vitro Amplification of DNA by the Polymerase 8 1 | 8 |
Preparation of Radiolabeled DNA and RNA Probes 9 1 | 9 |
INTRODUCTION | 3 |
Chapter 4 | 4 |
PROTOCOLS | 16 |
16-13 | |
16-21 | |
16-43 | |
17-1 | |
17-3 | |
17-5 | |
Transcriptional Runon Assays 17 23 | 17-23 |
INFORMATION PANELS | 17-75 |
INFORMATION PANELS | 17-91 |
INFORMATION PANELS | 17-99 |
Chapter 18 | 18-3 |
Extraction Purification and Analysis of mRNA from 7 1 | 18-7 |
Chapter 10 | 18-10 |
Chapter 14 | 18-14 |
INFORMATION PANELS | 18-82 |
INFORMATION PANELS | 18-107 |
INFORMATION PANELS | 18-115 |
Appendices | 18-137 |
Chapter 13 | 13 |
1 | 30 |
INFORMATION PANELS | 6 |
Chapter 8 | 8 |
Working with Highcapacity Vectors 4 1 | 4 |
INTRODUCTION | 10 |
DNA Sequencing 12 1 | 12 |
Working with Synthetic Oligonucleotide Probes 10 1 | 10 |
INTRODUCTION | R-20 |
1 | i |
PREFACE XV | xv |
Chapter 2 | 2 |
Screening Expression Libraries 14 1 | 14 |
1 | 56 |
Chapter 4 | 59 |
7 | 75 |
11 | 84 |
INFORMATION PANELS | 109 |
Amplifiers | 144 |
AnalogtoDigital Converters | 155 |
References | 165 |
CALIBRATION AND RESPONSE 93 | 173 |
577 | 183 |
59599 | 203 |
Other editions - View all
Molecular Cloning: A Laboratory Manual, Volume 1 Joseph Sambrook,David William Russell Limited preview - 2001 |