Molecular Cloning: A Laboratory Manual, Volume 1The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity. |
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Results 1-5 of 31
... Containing an IPTG - inducible Promoter ( Protocol 1 ) Several different vectors based on the lac operon are used for high - level expression of foreign pro- teins in E. coli , including : • The trp - lac ( tac ) promoter . tac is a ...
... containing the λ p , promoter to one containing the T7 promoter . Although the results of reengineering the protein are gratifyingly logical , it is impossible to understand why the expression system should dramatically influence the ...
... Containing Inclusion Bodies Lysis of bacteria containing inclusion bodies can generally be accomplished by standard methods , presented in Table 15-4 . The choice among these methods is usually dictated by the range of equipment that is ...
... containing SDS < ! > For the preparation of SDS - polyacrylamide gels used in the separation of proteins , please see Appendix 8 . Nucleic Acids and Oligonucleotides Gene or cDNA fragment of interest Media LB agar plates containing 50 ...
... containing the cDNA / gene of interest into the expression vector ( Chapter 1 , Protocol 17 or 19 ) . 3. Transform an E. coli strain containing the lac1a allele with the recombinant plasmid . If the plasmid vector itself carries the ...
Contents
15-3 | |
15-12 | |
15-49 | |
15-55 | |
15-64 | |
16-6 | |
16-7 | |
16-12 | |
Chapter 12 | 12 |
Chapter 9 | 11 |
1 | 13 |
In Vitro Amplification of DNA by the Polymerase 8 1 | 8 |
Preparation of Radiolabeled DNA and RNA Probes 9 1 | 9 |
INTRODUCTION | 3 |
Chapter 4 | 4 |
PROTOCOLS | 16 |
16-13 | |
16-21 | |
16-43 | |
17-1 | |
17-3 | |
17-5 | |
Transcriptional Runon Assays 17 23 | 17-23 |
INFORMATION PANELS | 17-75 |
INFORMATION PANELS | 17-91 |
INFORMATION PANELS | 17-99 |
Chapter 18 | 18-3 |
Extraction Purification and Analysis of mRNA from 7 1 | 18-7 |
Chapter 10 | 18-10 |
Chapter 14 | 18-14 |
INFORMATION PANELS | 18-82 |
INFORMATION PANELS | 18-107 |
INFORMATION PANELS | 18-115 |
Appendices | 18-137 |
Chapter 13 | 13 |
1 | 30 |
INFORMATION PANELS | 6 |
Chapter 8 | 8 |
Working with Highcapacity Vectors 4 1 | 4 |
INTRODUCTION | 10 |
DNA Sequencing 12 1 | 12 |
Working with Synthetic Oligonucleotide Probes 10 1 | 10 |
INTRODUCTION | R-20 |
1 | i |
PREFACE XV | xv |
Chapter 2 | 2 |
Screening Expression Libraries 14 1 | 14 |
1 | 56 |
Chapter 4 | 59 |
7 | 75 |
11 | 84 |
INFORMATION PANELS | 109 |
Amplifiers | 144 |
AnalogtoDigital Converters | 155 |
References | 165 |
CALIBRATION AND RESPONSE 93 | 173 |
577 | 183 |
59599 | 203 |
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Molecular Cloning: A Laboratory Manual, Volume 1 Joseph Sambrook,David William Russell Limited preview - 2001 |