Protein Expression in Animal CellsCritically acclaimed for more than 25 years, the Methods in Cell Biology series provides an indispensable tool for the researcher. Each volume is carefully edited by experts to contain state-of-the-art reviews and step-by-step protocols. Techniques are described completely so that methods are made accessible to users.
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Page viii
... Secretory Pathways R. A. Chavez, Y.-T. Chen, W. K. Schmidt, L. Carnell, and Hsiao-Ping Moore I. Introduction II. Considerations for the Choice of Expression Systems III. Plasmid-Based Expression IV. Vaccinia Virus-Based Expression V ...
... Secretory Pathways R. A. Chavez, Y.-T. Chen, W. K. Schmidt, L. Carnell, and Hsiao-Ping Moore I. Introduction II. Considerations for the Choice of Expression Systems III. Plasmid-Based Expression IV. Vaccinia Virus-Based Expression V ...
Page xv
... secretion. A chapter is included that describes the use of homologous recombination to create cultures of embryonic fibroblasts derived from homozygous mice with gene replacements or knockouts. Such cells can be used for experiments ...
... secretion. A chapter is included that describes the use of homologous recombination to create cultures of embryonic fibroblasts derived from homozygous mice with gene replacements or knockouts. Such cells can be used for experiments ...
Page 13
... secretory pathway, and was found to be sorted into a compartment that was distinct from condensed secretory proteins in murine pituitary cells (Tooze et al., 1987). Viruses of various other families are assembled at the plasma membranes ...
... secretory pathway, and was found to be sorted into a compartment that was distinct from condensed secretory proteins in murine pituitary cells (Tooze et al., 1987). Viruses of various other families are assembled at the plasma membranes ...
Page 38
... secretory proteins in primary structure and function. J. Biol. Chem. 253, 8667–8670, Lyles, D. S. (1979). Glycoproteins of Sendai virus are transmembrane proteins. Proc. Natl. Acad. Sci. USA 66, 5621–5625. Machamer, C., and Rose, J. K. ...
... secretory proteins in primary structure and function. J. Biol. Chem. 253, 8667–8670, Lyles, D. S. (1979). Glycoproteins of Sendai virus are transmembrane proteins. Proc. Natl. Acad. Sci. USA 66, 5621–5625. Machamer, C., and Rose, J. K. ...
Page 40
... secretion of rotavirus VP7, a resident endoplasmic reticulum membrane protein. J. Cell Biol. 101, 2199–2209. Racaniello, V. R. (1988). Poliovirus neurovirulence. Adv. Virus Res. 34, 217–246. Ren, R., and Racaniello, V. R. (1992). Human ...
... secretion of rotavirus VP7, a resident endoplasmic reticulum membrane protein. J. Cell Biol. 101, 2199–2209. Racaniello, V. R. (1988). Poliovirus neurovirulence. Adv. Virus Res. 34, 217–246. Ren, R., and Racaniello, V. R. (1992). Human ...
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Common terms and phrases
Acad acid adenovirus amplicon antibodies assay buffer cDNA CEFs Cell Biol Cell Biology cell lines cell surface cell types cellular centrifugation cloning containing cytoplasmic detected diluted dish DMEM efficiency encoding epithelial cells expression system expression vectors fibroblasts filter flask foreign gene function fusion protein gene expression gene gun gene transfer genetic genome glucose glycoproteins Golgi helper virus hemagglutinin herpes simplex virus heterologous hormone hr postinfection incubator infected cells influenza virus inserted labeling levels mammalian cells MDCK cells medium method microprojectiles Molecular monolayers mouse mRNA mutant Natl neurons NT2N cells pathway plaques plasma membrane plasmid plate polarized Proc promoter protein expression protocol pseudotyped receptor recombinant virus regulated replication resuspended retroviral vectors secretory sequence Sindbis virus studies supernatant targeting tissue culture titer transcription transfection transport trypsin vaccinia virus viral vectors virions Virol Virology virus stocks viruses vitro vivo wild-type
Popular passages
Page 287 - Greene, LA, and Tischler, AS (1976). Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.
Page 189 - Association between an oncogene and an anti-oncogene: the adenovirus E1A proteins bind to the retinoblastoma gene product. Nature (Lond.), 334: 124—129, 1988.
Page 189 - Wu. GY and Wu. CH (1987). Receptor-mediated in vitro gene transformation by a soluble DNA carrier system. J. Biol. Chem., 262: 4429-4432.
Page 287 - AJ (1973) A new technique for the assay of infectivity of human adenovirus 5 DNA.
Page 37 - Hogle, JM, Chow, M., and Filman, DJ (1985). Three-dimensional structure of poliovirus at 2.9 A resolution.
Page 52 - Birnboim, HC and Doly, J. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
Page 37 - Gibson, R., Schlesinger, S., and Kornfeld, S. (1979). The nonglycosylated glycoprotein of vesicular stomatitis virus is temperature-sensitive and undergoes intracellular aggregation at elevated temperatures. J. Biol. Chem. 254, 3600-3607.
Page 37 - M. (1973) Trypsin action on the growth of Sendai virus in tissue culture cells. III. Structural differences of Sendai viruses grown in eggs and tissue culture cells.
Page 352 - Ruley, HE (1983) Adenovirus early region 1A enables viral and cellular transforming genes to transform primary cells in culture.