Sample Preparation Techniques in Analytical ChemistrySomenath Mitra The importance of accurate sample preparation techniques cannot be overstated--meticulous sample preparation is essential. Often overlooked, it is the midway point where the analytes from the sample matrix are transformed so they are suitable for analysis. Even the best analytical techniques cannot rectify problems generated by sloppy sample pretreatment. Devoted entirely to teaching and reinforcing these necessary pretreatment steps, Sample Preparation Techniques in Analytical Chemistry addresses diverse aspects of this important measurement step. These include: * State-of-the-art extraction techniques for organic and inorganic analytes * Sample preparation in biological measurements * Sample pretreatment in microscopy * Surface enhancement as a sample preparation tool in Raman and IR spectroscopy * Sample concentration and clean-up methods * Quality control steps Designed to serve as a text in an undergraduate or graduate level curriculum, Sample Preparation Techniques in Analytical Chemistry also provides an invaluable reference tool for analytical chemists in the chemical, biological, pharmaceutical, environmental, and materials sciences. |
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Page 4
... amount of labor, time required to perform the analysis, and degree of automation can also be important. ysis, require calibration against chemical standards. The relationship between a detector signal and the amount of analyte is ...
... amount of labor, time required to perform the analysis, and degree of automation can also be important. ysis, require calibration against chemical standards. The relationship between a detector signal and the amount of analyte is ...
Page 5
... amount of analyte in the unknown samples. The calibration can be done in two ways, best illustrated by an example. Let us say that the amount of lead in soil is being measured. The analytical method includes sample preparation by acid ...
... amount of analyte in the unknown samples. The calibration can be done in two ways, best illustrated by an example. Let us say that the amount of lead in soil is being measured. The analytical method includes sample preparation by acid ...
Page 6
... amount of error, and an estimate of its magnitude is necessary to validate results. The error cannot be eliminated completely, although its magnitude and nature can be characterized. It can also be reduced with improved techniques. In ...
... amount of error, and an estimate of its magnitude is necessary to validate results. The error cannot be eliminated completely, although its magnitude and nature can be characterized. It can also be reduced with improved techniques. In ...
Page 21
... amount of this suspension between two KBr or NaCl plates. The edges of the plates are then sealed with soft wax. For the XPS measurements, the powder is spread on a tape attached to the sample holder and inserted into a transfer tube of ...
... amount of this suspension between two KBr or NaCl plates. The edges of the plates are then sealed with soft wax. For the XPS measurements, the powder is spread on a tape attached to the sample holder and inserted into a transfer tube of ...
Page 24
... amount of a sorbent and loaded with the sample extract. Elution of the analytes is e¤ected with a suitable solvent, leaving the interfering compounds on the column. The packing material may be an inorganic substance such as Florisil ...
... amount of a sorbent and loaded with the sample extract. Elution of the analytes is e¤ected with a suitable solvent, leaving the interfering compounds on the column. The packing material may be an inorganic substance such as Florisil ...
Contents
1 | |
SECTION A EXTRACTION AND ENRICHMENT IN SAMPLE PREPARATION | 37 |
CHAPTER 3 EXTRACTION OF SEMIVOLATILE ORGANIC COMPOUNDS FROM SOLID MATRICES | 139 |
CHAPTER 4 EXTRACTION OF VOLATILE ORGANIC COMPOUNDS FROM SOLIDS AND LIQUIDS | 183 |
CHAPTER 5 PREPARATION OF SAMPLES FOR METALS ANALYSIS | 227 |
SECTION B SAMPLE PREPARATION FOR NUCLEIC ACID ANALYSIS | 271 |
CHAPTER 7 SAMPLE PREPARATION IN RNA ANALYSIS | 301 |
CHAPTER 8 TECHNIQUES FOR THE EXTRACTION ISOLATION AND PURIFICATION OF NUCLEIC ACIDS | 331 |
SECTION C SAMPLE PREPARATION IN MICROSCOPY AND SPECTROSCOPY | 377 |
CHAPTER 10 SURFACE ENHANCEMENT BY SAMPLE AND SUBSTRATE PREPARATION TECHNIQUES IN RAMAN AND INFRARED SPEC... | 413 |
INDEX | 439 |
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Common terms and phrases
acid added addition allows amount Anal analysis analyte applications approach aqueous bonded bu¤er cell centrifugation Chem chemical chromatography coating column common complex compounds concentration containing depends described detection determined di¤erent digestion dissolved e¤ect electron elution ethanol example extraction fiber Figure followed groups headspace heating hydrophobic important increases injection interactions involves isolation layer less limit liquid materials matrix measurement membrane metals method minutes molecules needs obtained organic particles permission phase phenol plasmid polar possible precipitation present pressure procedure produce Raman range reaction recovery reduced referred relatively removed retained sample preparation selective separation shown silica soil solid solubility solution solvent sorbent species specimen SPME standard step surface Table techniques temperature tion tissue transfer trap tube typically vapor volatile volume
Popular passages
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