Sample Preparation Techniques in Analytical ChemistrySomenath Mitra The importance of accurate sample preparation techniques cannot be overstated--meticulous sample preparation is essential. Often overlooked, it is the midway point where the analytes from the sample matrix are transformed so they are suitable for analysis. Even the best analytical techniques cannot rectify problems generated by sloppy sample pretreatment. Devoted entirely to teaching and reinforcing these necessary pretreatment steps, Sample Preparation Techniques in Analytical Chemistry addresses diverse aspects of this important measurement step. These include: * State-of-the-art extraction techniques for organic and inorganic analytes * Sample preparation in biological measurements * Sample pretreatment in microscopy * Surface enhancement as a sample preparation tool in Raman and IR spectroscopy * Sample concentration and clean-up methods * Quality control steps Designed to serve as a text in an undergraduate or graduate level curriculum, Sample Preparation Techniques in Analytical Chemistry also provides an invaluable reference tool for analytical chemists in the chemical, biological, pharmaceutical, environmental, and materials sciences. |
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Page 4
... Analytes Sample Preparation Instrumenta Organics Extraction, concentration, cleanup, derivatization GC, HPLC, GC/MS ... analyte is obtained by recording the response from known quantities. Similarly, if an extraction step. 1.1.2. Methods ...
... Analytes Sample Preparation Instrumenta Organics Extraction, concentration, cleanup, derivatization GC, HPLC, GC/MS ... analyte is obtained by recording the response from known quantities. Similarly, if an extraction step. 1.1.2. Methods ...
Page 5
... analyte to the matrix and measure its recovery. Such processes require standards, which may be prepared in the laboratory or obtained from a commercial source. An important consideration in the choice of standards is the matrix. For ...
... analyte to the matrix and measure its recovery. Such processes require standards, which may be prepared in the laboratory or obtained from a commercial source. An important consideration in the choice of standards is the matrix. For ...
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... analyte from interferances 7 Speed of analysis Time needed for sample preparation and analysis 8 Throughput Number of samples that can be run in a given time period 9 Ease of automation How well the system can be automated 10 Ruggedness ...
... analyte from interferances 7 Speed of analysis Time needed for sample preparation and analysis 8 Throughput Number of samples that can be run in a given time period 9 Ease of automation How well the system can be automated 10 Ruggedness ...
Page 17
... analyte concentrations and in trace analysis. The sample collected is exposed to conditions di¤erent from the original source. For example, analytes in a groundwater sample that have never been exposed to light can undergo significant ...
... analyte concentrations and in trace analysis. The sample collected is exposed to conditions di¤erent from the original source. For example, analytes in a groundwater sample that have never been exposed to light can undergo significant ...
Page 19
... Analytes with high vapor pressures, such as volatile organics and dissolved gases (e.g., HCN, SO2) can easily be lost ... analyte. The surfaces can provide catalysts (e.g., metals) for reactions or just sites for irreversible adsorption ...
... Analytes with high vapor pressures, such as volatile organics and dissolved gases (e.g., HCN, SO2) can easily be lost ... analyte. The surfaces can provide catalysts (e.g., metals) for reactions or just sites for irreversible adsorption ...
Contents
1 | |
SECTION A EXTRACTION AND ENRICHMENT IN SAMPLE PREPARATION | 37 |
CHAPTER 3 EXTRACTION OF SEMIVOLATILE ORGANIC COMPOUNDS FROM SOLID MATRICES | 139 |
CHAPTER 4 EXTRACTION OF VOLATILE ORGANIC COMPOUNDS FROM SOLIDS AND LIQUIDS | 183 |
CHAPTER 5 PREPARATION OF SAMPLES FOR METALS ANALYSIS | 227 |
SECTION B SAMPLE PREPARATION FOR NUCLEIC ACID ANALYSIS | 271 |
CHAPTER 7 SAMPLE PREPARATION IN RNA ANALYSIS | 301 |
CHAPTER 8 TECHNIQUES FOR THE EXTRACTION ISOLATION AND PURIFICATION OF NUCLEIC ACIDS | 331 |
SECTION C SAMPLE PREPARATION IN MICROSCOPY AND SPECTROSCOPY | 377 |
CHAPTER 10 SURFACE ENHANCEMENT BY SAMPLE AND SUBSTRATE PREPARATION TECHNIQUES IN RAMAN AND INFRARED SPEC... | 413 |
INDEX | 439 |
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Common terms and phrases
acid added addition allows amount Anal analysis analyte applications approach aqueous bonded bu¤er cell centrifugation Chem chemical chromatography coating column common complex compounds concentration containing depends described detection determined di¤erent digestion dissolved e¤ect electron elution ethanol example extraction fiber Figure followed groups headspace heating hydrophobic important increases injection interactions involves isolation layer less limit liquid materials matrix measurement membrane metals method minutes molecules needs obtained organic particles permission phase phenol plasmid polar possible precipitation present pressure procedure produce Raman range reaction recovery reduced referred relatively removed retained sample preparation selective separation shown silica soil solid solubility solution solvent sorbent species specimen SPME standard step surface Table techniques temperature tion tissue transfer trap tube typically vapor volatile volume
Popular passages
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